乌珠穆沁羊肌球蛋白与MYHC相关基因的变化分析  

MYOSIN AND MYOSIN HEAVY CHAIN ISOFORM GENES CHANGES DURING THE GROWTH OF UJUMQIN SHEEP

作　　者：薛宝玲 宋晓彬 格日勒图[3] XUE Baoling;SONG Xiaobin;Gerilrtu(Vocational and Technical College of Inner Mongolia Agriculture University.Baotou 014109,China;Ulanqab Food and Drug Inspection Institute.Ulanqab 012000,China;College of Technical,Inner Mongolia Agriculture University.Hohhot 010010,China)

机构地区：[1]内蒙古农业大学职业技术学院食品工程技术系,包头014109 [2]内蒙古乌兰察布市食品药品检验所,乌兰察布012000 [3]内蒙古农业大学食品科学与工程学院,呼和浩特010010

出　　处：《内蒙古农业大学学报（自然科学版）》2020年第2期44-47,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

摘　　要：选取自然放牧条件下不同月龄乌珠穆沁羊的股二头肌为研究对象,采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法对MyHC异构体进行分离,利用PCR技术测定MyHC相关基因的相对表达量。结果表明:乌珠穆沁羊股二头肌中除了12月龄,MyHCI型基因随着月龄的增加其相对表达量高于MyHCIIb型基因的相对表达量,并且MyHCI和MyHCIIa型基因的相对表达量总和都要高于MyHCIIb型基因的相对表达量。MyHCI在9月龄和12月龄之间的差异较小,6月龄到9月龄期间变化较大。通过本研究探讨了乌珠穆沁羊随月龄的增加肌球蛋白重链相关基因的变化规律。The biceps femoris of different aged Ujumqin sheeps that were rose under the condition of natural pasture were research in this study.MyHCs were separated by SDS-PAGE.The expression level of MyHC genes were investigated by Real-time PCR.The relative expression of MyHCI gene in biceps femoris was higher than MyHCIIb gene of Ujumqin sheeps except 12 months old.The sum of expression of MyHCI and MyHCIIa genes was higher than MyHCIIb genes.The small difference of MyHCI between 9-12-month-old,and the significant difference between 6-month-old and 9-month-old.The rule of MyHCs changing with month was analyzed in the study,which had important significant to processing of animal products.

关 键 词：乌珠穆沁羊 肌球蛋白重链基因 肌球蛋白 

分 类 号：TS251.2[轻工技术与工程—农产品加工及贮藏工程]