RNA-Seq Study Reveals AP2-Domain-Containing Signalling Regulators Involved in Initial Imbibition of Seed Germination in Rice  

作　　者：HE Yongqi ZHAO Jia FENG Defeng HUANG Zhibo LIANG Jiaming ZHENG Yufei CHENG Jinping YING Jifeng WANG Zhoufei 

机构地区：[1]Laboratory of Seed Science and Technology/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou 510642,China [2]Laboratory of Seed Science and Technology,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,China [3]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Rice science》2020年第4期302-314,共13页水稻科学（英文版）

基　　金：supported by the National Key Research and Development Plan (Grant No. 2018YFD0100901);the Guangdong Province Key Research and Development Program (Grant No. 2018B020202012);the Guangdong Province Key Laboratory of Plant Molecular Breeding (Grant No. GPKLPMB201903);the Major Scientific Research Projects of General Colleges and Universities of Guangdong Province (Grant No. 2017KTSCX024)

摘　　要：A number of internal signals are required for seed germination.However,the precise signalling responses in the initial imbibition of seed germination are not yet fully understood in rice.In this study,the RNA sequencing(RNA-Seq)approach was conducted in 8 h imbibed seeds to understand the signalling responses in the initial imbibition of rice seed germination.A total of 563 differentially expressed genes(DEGs)with at least 4-fold change were identified in 8 h imbibed seeds compared to dry seeds.MapMan analysis revealed that the majority of signalling response-related DEGs were hormone-and transcription factor-related genes,in which the largest number of DEGs belong to the AP2-domain-containing regulators,and their expressions were significantly induced in the initial imbibition of seed germination in rice.Moreover,at least five AP2-domain-containing transcription factor OsDREBs were identified in the initial imbibition of rice seed germination,and the expressions of 251 DEGs were putatively regulated by OsDREBs through the dehydration-responsive element(DRE)cis-element assay.It suggested that the OsDREBs might play important roles in the regulation of initial seed imbibition in rice.The identified genes provide a valuable resource to study the signalling regulation of seed germination in the future.

关 键 词：RICE seed imbibition signalling response plant hormone transcription factor RNA-sequencing 

分 类 号：S511[农业科学—作物学]