长时程抑郁小鼠模型建立及行为与神经病理特征观察  

Establishing a long-term depression model in mice;behavior and neuropathological features

作　　者：田野[1] 栗鋆 原一桐 杜若琛 李宵[1] 王菲[1] 王雅丽[1] 邵宏元[2] 王春芳[1] TIAN Ye;LI Yun;YUAN Yitong;DU Ruochen;LI Xiao;WANG Fei;WANG Yali;SHAO Hongyuan;WANG Chunfang(Shanxi Medical University,Taiyuan 030001,China;Shanxi Provincial People’s Hospital,Taiyuan 030001)

机构地区：[1]山西医科大学,太原030001 [2]山西省人民医院,太原030001

出　　处：《中国实验动物学报》2020年第3期358-363,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(81371384);山西省重点研发项目(201803D31068);山西省高等学校科技创新项目(2019L0418,2019L0445)。

摘　　要：目的构建新型长时程抑郁模型(long-term depression model,LTD)小鼠,并对比此种小鼠与常见抑郁小鼠模型的优劣。方法通过早期母婴分离,以及断乳后持续慢性应激来构建LTD小鼠,对比其与慢性不可预知温和刺激模型(chronic unpredictable wild stress,CUMS)、早期应激模型(early life stress model,ELS)小鼠长期抑郁样行为改变,并对比e GFP转基因的LTD小鼠与对照小鼠海马神经元变化。结果与CUMS、ELS小鼠相比,LTD小鼠在行为学上能更长期的表现出稳定的抑郁状态,组织切片显示LTD小鼠海马CA1区和CA3区呈退行性变性。结论成功的构建了LTD小鼠,此模型小鼠能导致更长久抑郁样行为,表现出长期的心理应激状态,是一种适合用于长期模拟抑郁状态的模式小鼠。Objective To establish a novel long-term depression(LTD)model in mice,and to compare this model with the common depression mouse model.Methods Using early maternal separation and persistent chronic stress after weaning to prepare LTD mice,which were then compared with chronic unpredictable mild stress and early life stress mice to observe potential differences in their long-term depression-like behaviors.The changes in hippocampal neurons of LTD mice expressing transgenic eGFP were also observed.Results Compared with chronic unpredictable mild stress and early life stress mice,LTD mice showed a stable state of depression in animal behavior experiments.Degeneration of the hippocampal CA1 and CA3 regions of LTD mice was observed in tissue sections.Conclusions The LTD mouse model was successfully established.LTD mice provide a model suitable for the investigation of long-term simulation of depression,which can lead to long-term depression-like behavior and psychological stress.

关 键 词：抑郁症 工具鼠 行为学 应激 

分 类 号：Q95-33[生物学—动物学]