盐酸洛哌丁胺诱导的小鼠慢传输型便秘模型的实验研究  

Experimental study of slow transit constipation induced by loperamide hydrochloride in mice

作　　者：姚一博[1] 王迪 王钱陶 肖长芳 梁宏涛[1] 曹永清[1] 陆金根[1] 王琛[1] YAO Yibo;WANG Di;WANG Qiantao;XIAO Changfang;LIANG Hongtao;CAO Yongqing;LU Jingen;WANG Chen(Longhua Hospital Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属龙华医院肛肠科,上海200032

出　　处：《中国实验动物学报》2020年第3期370-375,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81603625,81603618,81603624);上海中医药大学杏林学者,全国名老中医药专家传承工作室建设项目(批文号:国中医药人教教育便函[2016]42号);曹永清上海市名老中医学术经验研究工作室SHGZS-2017017。

摘　　要：目的通过盐酸洛哌丁胺诱导慢传输型便秘小鼠模型,比较不同剂量、不同给药时间所诱导模型之间的差异,探索建立稳定的盐酸洛哌丁胺诱导的慢传输便秘小鼠模型各项实验参数。方法采用健康雄性C57BL/6小鼠,配制0.025、0.25和2.5 mg/m L的盐酸洛哌丁胺混悬液灌胃,给药时间为每日上午9时和下午3时,分两次给药。从肠道推进率检测、排便量检测等指标进行造模效果评价,观察不同剂量、不同给药时间对于慢传输型便秘小鼠模型的影响。结果 1)对比不同剂量的盐酸洛哌丁胺造模的效果,在0.5 mg/kg^50 mg/kg的给药浓度范围内,通过比较组间肠道推进率,结果表明50 mg/kg组与空白组相比,小鼠肠道推进率显著降低、排便量明显减少,差异具有统计学意义(P<0.05);(2)对比3、7、10和14 d四个不同造模天数,造模第7天时,小鼠的肠道推进率与空白组相比明显降低(P<0.05);而3、10、14 d肠道推进率相比无明显变化(P>0.05)。结论本次研究发现采用50 mg/kg的剂量连续灌胃7 d,可使小鼠的肠道推进率显著降低,排便量明显减少,能够制造相对稳定的结肠传输障碍性便秘小鼠模型,为盐酸洛哌丁胺诱导慢传输型便秘模型的探索提供了有价值的数据资料。Objective To find a stable method for establishing a loperamide hydrochloride-induced slow transit constipation(STC)model by comparing the differences among STC mouse models established by different loperamide hydrochloride concentrations and administration times.Methods Healthy male C57BL/6 mice were given 0.025,0.25 or 2.5 mg/mL loperamide hydrochloride by intragastric administration twice a day at 9:00 and 15:00.The small intestinal propulsion rate,defecation quantity and other indicators were used to evaluate the effects of different doses and administration times on the STC mouse model.Results(1)Comparing the effects of different dosages of loperamide hydrochloride in the range of 0.5 mg/kg to 50 mg/kg revealed that the small intestinal propulsion rate and defecation quantity significantly decreased in the 50 mg/kg dosage group compared with those in the control group(P<0.05).(2)At day 7 after oral administration of loperamide hydrochloride,the small intestinal propulsion rate significantly decreased compared with that in control group(P<0.05),whereas this rate did not significantly change at days 3,10 and 14(P>0.05).Conclusions The small intestinal propulsion rate and defecation quantity significantly decreased after continuous administration of 50 mg/kg loperamide hydrochloride for 7 days.Hence,this is a relatively stable method for establishing an STC mouse model.This study provides valuable data for the exploration of the loperamide hydrochloride-induced STC model.

关 键 词：盐酸洛哌丁胺 慢传输型便秘 小鼠模型 

分 类 号：Q95-33[生物学—动物学]