High-resolution linkage and quantitative trait locus mapping using an interspecific cross between Argopecten irradians irradians(♀) and A. purpuratus (♂)  

作　　者：Junxia Mao Qifan Zeng Zujing Yang Haoyuan Pan Lijie Yao Zhenmin Bao Chunde Wang Shi Wang 

机构地区：[1]MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [2]Key Laboratory of Mariculture and Stock Enhancement in North China's Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China [3]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China [4]Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China [5]Marine Science and Engineering College,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Marine Life Science & Technology》2020年第2期123-134,共12页海洋生命科学与技术（英文）

基　　金：the Grant support from National Natural Science Foundation of China(U1706203,31172404 and 31572618);Taishan Scholar Project Fund of Shandong Province of China,and Youth Talent Program Supported by Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao)(2018-MFS-T07).

摘　　要：The bay scallop and Peruvian scallop are economically important species.Interspecific hybrids of these two scallops outperformed both of their parent species in multiple growth traits but exhibited decreased fertility,which provides good models for the study of heterosis and species divergence.Genetic mapping serves as a chromosomal-level framework to investigate the molecular mechanisms of hybridization and introgression.In this study,high-resolution linkage maps were constructed for the bay and Peruvian scallops with an interspecific hybrid family.The linkage map of the bay scallop covered over 98.9% of the whole genome with 2994 mapped markers and the average marker interval of 0.32 cM.For the Peruvian scallop,1585 markers were mapped with the average maker interval of 0.51 cM,covering 97.7% of the genome.Both the two linkage maps have 16 linkage groups,corresponding to the haploid chromosome number of the two species.Approximately,54.5% of markers exhibited significant deviation from the expected Mendelian ratio of segregation,lending in sights into the intrinsic incompatibility between the two species.QTLs related to growth and shell coloration were detected,which could explain 13.1%and 74.9% of the phenotypic variance,respectively.This represents important information for further evaluation.These findings are an important addition to the genomic resources for scallop genetic studies,and are especially useful for investigations on genomic incompatibility for hybridization,genome evolution of closely related species,and genetic enhancement programs in aquaculture.

关 键 词：SCALLOP Genetic map 2b-RAD genotyping Quantitative trait locus mapping Interspecific hybridization 

分 类 号：S917.4[农业科学—水产科学]