五味子丙素缓解血管紧张素Ⅱ诱导的心脏炎性反应和心肌重构  

Schisandrin C alleviates AngⅡ-induced cardiac inflammation and myocardial remodeling

作　　者：谈云 叶琳岚 赵珺瑶 吕卫群[1] TAN Yun;YE Linlan;ZHAO Junyao;LYU Weiqun(Department of Phamacy,The Third People’s Hospital of Wuxi City(Affiliated Hospital of Jiangnan University),Wuxi,Jiangsu 214041,China)

机构地区：[1]无锡市第三人民医院(江南大学附属医院)药剂科,江苏无锡214041

出　　处：《安徽医药》2020年第7期1291-1295,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究五味子丙素对血管紧张素Ⅱ(AngⅡ)诱导小鼠心肌重构和炎性反应的影响。方法研究时间为2018年7月至2019年1月。皮下注射AngⅡ诱导C57BL/6小鼠心肌重构。将C57BL/6小鼠分为空白对照组、AngⅡ组、AngⅡ+五味子丙素组(AngⅡ+ShC组),皮下注射AngⅡ前一天开始给药处理,8周后,处死小鼠,收集心脏组织。采用ELISA法检测组织中肿瘤坏死因子-α(TNF-α)/白介素-6(IL-6)的含量,RT-PCR法检测IL-6、TNF-α、心钠肽(ANP)、脑钠肽(BNP)、肌球蛋白重链(MYHC)、转化生长因子β(TGF-β)、胶原IV(Collagen IV)和β-肌动蛋白(β-actin)的mRNA表达;生化试剂盒检测血液中的CK-MB。结果与AngⅡ组比较,五味子丙素治疗组心脏损伤被逆转,心重比降低[(6.32±0.99)‰比(4.93±0.17)‰,P<0.05],心功能相关基因ANP[(3.37±0.60)比(1.41±0.37)倍,P<0.05)和BNP[(2.51±0.75)比(0.78±0.55)倍,P<0.05]、促肥大基因MYHC[(2.57±0.74)比(1.18±0.26)倍,P<0.05)、纤维化相关基因TGF-β[(5.25±0.94)比(1.78±0.57)倍,P<0.05)和胶原IV[(3.70±1.47)比(1.68±0.56)倍,P<0.05]的mRNA表达下调,心脏组织TNF-α/IL-6的mRNA[分别为(7.18±2.85)比(2.92±0.86)倍,P<0.05和(12.89±5.40)比(4.29±3.43)倍,P<0.05]和蛋白表达水平[分别为(4.80±0.78)比(2.09±0.41)ng/mg,P<0.05和(2.56±0.73)比(1.23±0.41)ng/mg,P<0.05]均显著降低。结论五味子丙素能有效缓解AngⅡ诱导的小鼠心肌重构,并伴随缓解炎性反应。Objective To investigate the effects of schisandrin C on alleviating myocardial remodeling and inflammation induced by angiotensinⅡ(AngⅡ)in mice.MethodsThe research period is from July 2018 to January 2019.AngⅡwas injected subcutane-ously to induce cardiac remodeling in C57 BL/6 mice.C57 BL/6 mice were divided into blank control group,AngⅡgroup and AngⅡ+schisandrin C group.The mice were subcutaneously injected with AngⅡone day before the drug treatment was started.8 weeks later,the mice were sacrificed and cardiac tissue was collected.The contents of tumor necrosis factor-α(TNF-α)/interleukin-6(IL-6)were detected by ELISA,the mRNA expressions of IL-6,TNF-α,ANP,BNP,MYHC,TGF-β,col-4 andβ-actin were detected by RT-PCR,and CK-MB in blood was detected by biochemical kit.ResultsCompared with AngⅡgroup,cardiac injury in the schisan-drin treatment group was reversed,the heart weight ratio decreased[(6.32±0.99‰)vs(.4.93±0.17‰),P<0.05],the expressions ofcardiac function related genes ANP[(3.37±0.60)vs.(1.41±0.37)times,P<0.05)and BNP(2.51±0.75)vs.0.78±0.55)times,P<0.05],hypertrophic gene MYHC[(2.57±0.74)vs.(1.18±0.26)times,P<0.05)and fibrosis related genesTGF-β[(5.25±0.94)vs.(1.78±0.57)times,P<0.05)and Collagen IV(3.70±1.47)vs.1.68±0.56)times,P<0.05]were decreased,and the m RNA[(7.18±2.85)vs.(2.92±0.86)times,P<0.05 and(12.89±5.40)vs.(4.29±3.43)times,P<0.05,respectively]and protein expression[(4.80±0.78)vs.2.09±0.41)ng/mg,P<0.05 and(2.56±0.73)vs.(1.23±0.41)ng/mg,P<0.05,respectively]of TNF-a/IL-6 in cardi-ac tissue decreased significantly.ConclusionSchisandrin C can effectively alleviate the myocardial remodeling induced by AngⅡin mice,accompanied by alleviating inflammatory response.

关 键 词：五味子 血管紧张素Ⅱ 肿瘤坏死因子α 白细胞介素6 心钠素 利钠肽 脑 肌球蛋白重链 转化生长因子β 心肌重构 炎症反应 

分 类 号：R285.5[医药卫生—中药学]