线粒体活性对人类精子活动力的影响  

Effects of mitochondrial activity on human spermatozoa motility

作　　者：张友旺 潘龙瑞 刘俊[2] 马开槐 张特 谭艳 Zhang Youwang;Pan Longrui;Liu Jun;Ma Kaihuai;Zhang Te;Tan Yan(Lab of Medical Functions,School of Basic Medical Science,Hubei University of Medicine;Blood vessels,Pediatric and Gastrointestinal(Ⅱ)Surgery,Tongren Hospital of Wuhan University;School of Basic Medical Science,Hubei University of Medicine;Biomedical Research Institute of Hubei University of Medicine;Department of Andrology,Renmin Hospital Affiliated to Hubei University of Medicine)

机构地区：[1]湖北医药学院基础医学院机能实验室,十堰442000 [2]武汉大学附属同仁医院血管、小儿及胃肠(Ⅱ)外科,武汉430073 [3]湖北医药学院基础医学院,十堰442000 [4]湖北医药学院生物医药研究院,十堰442000 [5]湖北医药学院附属人民医院男科,十堰442000

出　　处：《重庆医科大学学报》2020年第5期580-584,共5页Journal of Chongqing Medical University

基　　金：湖北医药学院人才启动金资助项目(编号:2017QDJZR21)。

摘　　要：目的:分析线粒体活性对人类精子活动力的影响。方法:以线粒体特异性荧光染料--mitotracker deep red 633(MTDR对不同活力的精子进行染色,然后用流式细胞术测定精子线粒体活性(即MTDR荧光强度值)。用雷帕霉素(rapamycin,RP)和羟氯喹(hydroxychloroquineon,HCQ)调控精子活性水平,测定精子活动力的变化。结果:MTDR荧光强度值与前向运动速率(progressive motility,PR)不相关(r=0.182,P=0.268)。5 nmol/L和100 nmol/L的RP均可明显降低线粒体活性,50μmol/L HCQ可明显提高线粒体活性(P<0.05),但它们对精子活动力影响都不明显(P>0.05)。结论:线粒体活性对人类精子活力影响不明显。Objective:To analyze effects of mitochondrial activity on human spermatozoa motility.Methods:Mitochondrial specific fluorescent dye,mitotracker deep red 633(MTDR),was used to stain sperms with different motility.And the flow cytometry was used to determine the activity of sperm mitochondria(that is,value of MTDR fluorescence intensity).Rapamycin(RP)and hydroxychloroquine(HCQ)were used to regulate the mitochondrial activity,and then measure the change of sperm motility.Results:MTDR fluorescence intensity value was not correlated with spermatozoa progressive motility(PR)rate(r=0.182,P=0.268).RP of 5 nmol/L or 100 nmol/L was able to significantly decrease the mitochondrial activity,and HCQ of 50μmol/L was able to significantly increase the mitochon-drial activity(P<0.05).However,those above concentrations had no obvious effect on sperm motility(P>0.05).Conclusion:There is no obvious effect of mitochondrial activity on human sperm motility.

关 键 词：mitotracker deep red 633 线粒体 精子 活动力 

分 类 号：R698.2[医药卫生—泌尿科学]