紫柏凝胶对宫颈癌裸鼠移植瘤生长及瘤组织E6、E7蛋白表达的影响  

Effect of Zibai Gel on Tumor Growth and Expression of E6 and E7 Protein in Cervical Mouse Transplant

作　　者：温利娟 薛晓鸥[1] 谢伟[1] WEN Li-juan;XUE Xiao-ou;XIE Wei(Department of Gynecology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing,100700)

机构地区：[1]北京中医药大学东直门医院妇科,北京100700

出　　处：《中国中西医结合杂志》2020年第6期721-726,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：北京市科技计划课题G20工程支撑保障项目(No.Z151100003815014)。

摘　　要：目的观察紫柏凝胶对宫颈癌裸鼠的抑瘤作用及对E6、E7、Rb、P53蛋白表达的影响。方法建立人宫颈癌Siha细胞荷瘤裸鼠模型,将成瘤裸鼠按随机数字表法分为模型组,顺铂组,紫柏凝胶高、中、低剂量组,每组7只。模型组不干预,顺铂组腹腔内注射0.5 mL顺铂(5 mg/kg),每4日1次,紫柏凝胶高、中、低剂量组分别在瘤体表面涂抹紫柏凝胶0.9428、0.4714、0.2357 g/kg,每日1次,连续用药28日。每日测量肿瘤体积、裸鼠重量、进食水量,停药24 h后处死裸鼠,完整取出肿瘤组织,称重并测量体积,将肿瘤组织切片后,行HE染色,行移植瘤组织病理学观察,并采用免疫组化SP的方法,检测肿瘤组织中E6、E7、Rb和P53蛋白表达水平。结果与模型组比较,紫柏凝胶中、高剂量组及顺铂组在治疗第22、28日肿瘤体积减小(P<0.05),瘤重降低(P<0.05),E6、Rb蛋白表达均降低(P<0.05),紫柏凝胶高剂量组及顺铂组E7、P53蛋白表达均降低(P<0.05)。与顺铂组比较,紫柏凝胶各剂量组瘤重升高(P<0.05),抑瘤率降低(P<0.05),E6、P53、E7、Rb蛋白表达均升高(P<0.05)。与紫柏凝胶低剂量组比较,紫柏凝胶中、高剂量组瘤重降低(P<0.05),抑瘤率升高(P<0.05),E6、P53、E7、Rb蛋白表达均降低(P<0.05)。结论紫柏凝胶对人宫颈癌Siha细胞移植瘤的生长具有明显抑制作用,其机制与下调致癌蛋白E6和E7的表达有关。Objective To observe anti-tumor effect of Zibai Gel on cervical cancer in nude mice and its effect on the expression of E6,E7,Rb and P53 proteins.Methods The nude mice bearing human cervical cancer Siha cells were established,and the tunor-formig nude mice were randomly divided into model group,cisplatin group,and high-,medium-,low-dose Zibai Gel groups,7 nude mice in each group.The cisplatin group was intraperitoneally injected with 0.5 mL cisplatin(5 mg/kg)once every 4 days.The high-,medium-,low-dose Zibai Gel groups were respectively coated with Zibai Gel 0.9428,0.4714,0.2357 g/kg on the tumor surface,once a day for 28 consecutive days.The tumor volume,nude mice weight,and water intake were measured daily.The nude mice were sacrificed at 24 h after drug withdrawal.The tumor tissues were completely removed,weighed and measured.The tumor tissues were sliced and then stained with HE.The protein expressions of E6,E7,RB and P53 in tumor tissues were detected by immunohistochemical SP method.Results Compared with model group,tumor volume was significantly decreased in medium-dose Zibai Gel group,high-dose Zibai Gel group,and cisplatin group at both 22 days and 28 days(P<0.05),the weight of tumors was significantly decreased(P<0.05),E6,Rb expression were decreased(P<0.05).E7,P53 expression were decreased in high-dose Zibai Gel group and cisplatin group(P<0.05).Compared with cisplatin group,the weight of tumors was significantly increased in various Zibai Gel groups(P<0.05),the tumor inhibitory rate was significantly decreased(P<0.05),E6,E7,P53 and Rb expression were increased(P<0.05).Compared with low-dose Zibai Gel group,the weight of tumors was significantly decreased in medium-dose Zibai Gel group and high-dose Zibai Gel group(P<0.05),the tumor inhibitory rates were increased(P<0.05),E6,E7,Rb and P53 expression were decreased(P<0.05).Conclusions Zibai Gel can significantly inhibit the growth of human cervical cancer by Siha cells transplantedin vitro.The mechanism is related to down-regulating E6 and E7 oncoprotei

关 键 词：紫柏凝胶 宫颈癌 人乳头瘤病毒 E6蛋白 E7蛋白 

分 类 号：R965[医药卫生—药理学]