高温和HgCl2复合处理对水稻花期生理特性和水通道蛋白基因表达的影响  

Effect of high temperature and HgCl2 stress on physiological parameters and aquaporins genes expression in rice flowering stage

作　　者：胡挺帅 郑梦琪 郑嘉琪 罗心怡 张志友 潘伟槐[1] 郭天荣[1] 莫亿伟[1] HU Tingshuai;ZHENG Mengqi;ZHENG Jiaqi;LUO Xinyi;ZHANG Zhiyou;PAN Weihuai;GUO Tianrong;MO Yiwei(College of Life Science,Shaoxing University,Shaoxing,Zhejiang 312000,China)

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000

出　　处：《湖南农业大学学报（自然科学版）》2020年第3期262-270,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31071353);浙江省自然科学基金项目(LY16C130004、LY14C130003);浙江省科技厅公益项目(LGN19C130003)。

摘　　要：以转OsPIN1a的基因水稻‘中花11’为材料,设置3个处理:适温处理(CK),昼夜温度为30℃/28℃;高温处理(TR1),昼夜温度为38℃/33℃;复合处理(TR2),昼夜温度为38℃/33℃,配以0.1 mmol/L HgCl2处理,研究高温胁迫和水通道蛋白抑制剂(HgCl2)对水稻花期生理特性、水通道蛋白基因表达、稻穗和剑叶相对含水量及淀粉粒发育的影响。结果表明:与适温处理相比,高温与HgCl2胁迫均能降低稻穗和剑叶相对含水量,降低结实率和千粒质量,降低柱头上花粉粒数量及柱头和花粉粒的线粒体活性,抑制OsTIPs和OsPIPs家族多个基因的表达,使稻穗水分供给减少,导致胚乳淀粉粒发育不良,形成不规则圆球形,以高温与HgCl2复合处理时影响最大。水通道蛋白基因家族表达受抑制后,加重了高温胁迫对水稻植株花期生长发育的伤害,致使花粉粒和柱头活性下降,导致结实率降低和籽粒充实不良。To explore the effects of high temperature and aquaporins inhibitor(HgCl2)treatments on the expression of aquaporins genes in flag leaves,the relative water content in panicle and flag leaves,and the development of rice endosperm,the‘over-expression OsPIN1a’rice was treated with 38℃/33℃and 0.1 mmol/L HgCl2 for 6 days during the flowering period.The main physiological parameters including relative water content,seed setting rate,1000-grains weight,endosperm cell structure,and expression of tonoplast intrinsic proteins(OsTIPs)and plasma membrane intrinsic proteins(OsPIPs)in ears or flag leaves were measured.Results showed that,compared to optimal temperature(30℃/28℃)treatment,high temperature or HgCl2 stress significantly decreased contents of relative water,seed setting rate and 1000-grain weight,activities of mitochondrial in pollen and stigma,pollen number in stigma,and expressions of OsTIPs and OsPIPs family genes.Moreover,starch accumulation in endosperm cells was delayed and irregular shape of its granules was observed.High temperature combined with HgCl2 stress induced more severe development inhibition and toxicity.In conclusion,lower expression of OsTIPs and OsPIPs family genes could aggravate the growth and the development inhibition induced by high temperature,and decreased the activity of pollen and stigmas,resulting in lower seed setting rate and poor grain filling.

关 键 词：水稻 高温胁迫 水通道蛋白抑制剂(HgCl2) 水通道蛋白基因表达 花期 生理特性 

分 类 号：S511.01[农业科学—作物学]