佛甲草醇提物对佐剂性关节炎大鼠脾脏炎症相关蛋白表达的影响  

The effects of Sedum lineare Thunb on inflammation-related protein expression in the spleen of adjuvant arthritis rats

作　　者：曹性玲[1] 吴丽珍[1] 麻海娟 曾丽梅[1] 周青[1] CAO Xing-ling;WU Li-zhen;MA Hai-juan;ZENG Li-mei;ZHOU Qing(School of Basic Medicine of Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院基础医学院,江西赣州341000

出　　处：《赣南医学院学报》2020年第5期477-481,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省卫生计生委中医药课题(NO.2014A097)。

摘　　要：目的:观察佛甲草醇提物对佐剂性关节炎大鼠细胞因子和炎症相关信号通路的作用。方法:制备佐剂性关节炎大鼠模型,Western blot方法检测佛甲草提取物对大鼠脾脏组织中TNF-α、IL-1β、IL-17、IL-10、NF-κB及a7nAChR的蛋白表达。结果:与模型组相比,4 g·kg^-1佛甲草治疗组TNF-α(P<0.01)、IL-1β(P<0.05)、IL-17(P<0.05)及NF-κB(P<0.05)蛋白表达降低,IL-10(P<0.01)及a7nAChR(P<0.01)蛋白表达升高。结论:佛甲草醇提物可能通过调控脾脏的促炎和抑炎症细胞因子及相关信号,调节机体的免疫功能,从而控制佐剂性关节炎诱导的急性和继发性关节炎。Objective:To observe the effects of Sedum lineare Thunb(SLT)on inflammatory factors and inflammatory related signaling pathways in Rheumatoid Arthritis(RA)rats.Methods:Preparation of Rheumatoid Arthritis Rats model,the expression of the TNF-α,IL-1β,IL-17,IL-10,NF-κB and a7nAChR in RA rats spleen were detected by Western blot assay.Results:Compared with the model group,the expression of TNF-α,IL-1β,IL-17,NF-κB were downregulated and the IL-10,a7nAChR were upregulated in SLT group.Conclusions:SLT can control acute and secondary arthritis induced by RA,the possible mechanism correlates with the regulation of pro-inflammatory and anti-inflammatory cytokines,and regulate the immune function.

关 键 词：佛甲草醇提物 佐剂性关节炎 NF-ΚB信号通路 炎症因子 

分 类 号：R285[医药卫生—中药学]