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作 者:刘广超[1] 田晓玲[1] 李秀阳 黄承玲 LIU Guang-chao;TIAN Xiao-ling;LI Xiu-yang;HUANG Cheng-ling(College of Humanities and Sciences,Guizhou Minzu University,Guiyang 550025,China;College of Eco-Environmental Engineering,Guizhou Minzu University,Guiyang 550025,China)
机构地区:[1]贵州民族大学人文科技学院,贵阳550025 [2]贵州民族大学生态环境工程学院,贵阳550025
出 处:《湖北农业科学》2020年第8期107-110,共4页Hubei Agricultural Sciences
基 金:贵州省科技合作计划项目(黔科合LH字[2016]7085)。
摘 要:为探究不同消毒方式和不同萌发介质对杜鹃属(Rhododendron L.)植物种子萌发的影响,以10种杜鹃属植物的种子为研究对象进行种子萌发试验,消毒方式分别为0.3%高猛酸钾溶液消毒10 min,1%次氯酸钠消毒10 min,0.1%升汞消毒10 min,70%乙醇消毒30 s+1%次氯酸钠消毒10 min,70%乙醇浸泡30 s+0.1%升汞消毒10 min;萌发介质分别为滤纸、海绵、纱布和琼脂培养基,比较不同消毒方式和萌发介质的种子发芽率、发芽势和发芽时滞。结果表明,不同消毒方式对不同杜鹃属种子发芽率和发芽势影响不同,1%NaClO和70%乙醇+1%NaClO消毒后的发芽率和发芽势较其他消毒方式高,且发芽时滞也明显短于其他消毒方式。琼脂培养基适合多数杜鹃种子的萌发,而纱布不适合作为杜鹃属种子的萌发介质。To explore the effects of different disinfection methods and germination matrix of rhododendron,10 species of Rhododendron L.were tested,disinfection treatment were as follows,disinfect with a concentration of 0.3%of the potassium acid solution for 10 minutes,disinfect with 1%sodium hypochlorite solution for 10 minutes,disinfect 10 min with 0.1%Hg solution,disinfect with 70%alcohol for 30 seconds+1%sodium hypochlorite for 10 minutes,disinfect with 70%alcohol for 30 seconds+0.1%Hg solution for 10 minutes.In addition,the germination rate,germination potential and germination time of 10 species of Rhododendron L.seeds were determined by filter paper,sponge,gauze and specific culture medium.The results showed that,different disinfection methods had different effects on germination rate and germination potential of different Rhododendron L..But on the whole,the germination rate and germination potential of 1%NaClO and 70%alcohol+1%NaClO were higher than other disinfection methods.And the delay of germination were also significantly shorter than other disinfection methods.Agar culture medium was suitable for most of the Rhododendron L.seed germination,and the gauze was not suitable for germination matrix of Rhododendron L.seed.
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