补肾解毒颗粒中4种成分测定及HPLC特征图谱建立  

Determination of four constituents and establishment of characteristic HPLC chromatograms for Bushen Jiedu Granules

作　　者：谭志滨 徐俊鸿[1] 邓卓燕 蓝海[1] TAN Zhi-bin;XU Jun-hong;DENG Zhuo-yan;LAN Hai(Shunde Hospital of Guangzhou University of Chinese Medicine,Foshan 528300,China)

机构地区：[1]广州中医药大学顺德医院,广东佛山528300

出　　处：《中成药》2020年第6期1426-1431,共6页Chinese Traditional Patent Medicine

基　　金：广东省科技发展计划项目(2016ZC0102)。

摘　　要：目的测定补肾解毒颗粒(熟地黄、山药、补骨脂等)中4种成分含有量,并建立HPLC特征图谱。方法该药物50%甲醇提取液的分析采用Thermo Acclaim TM 120 C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;检测波长240、246 nm;柱温35℃。结果莫诺苷、马钱苷、补骨脂素、异补骨脂分别在2.481 8~124.087 6、2.328 5~116.424 0、1.064 8~53.239 8、1.528 3~76.416 0μg/mL范围内线性关系良好(r≥0.999 9),平均加样回收率分别为102.43%、98.61%、92.02%、96.93%,RSD分别为3.75%、5.79%、3.46%、5.80%。15批样品特征图谱中有6个特征峰,相似度0.997~0.999。结论该方法简便可靠,重复性好,可用于补肾解毒颗粒的质量控制。AIM To determine the contents of four constituents in Bushen Jiedu Granules(Rehmanniae Radix Praeparata, Dioscoreae Rhizoma, Psoraleae Fructus, etc.) and to establish their characteristic HPLC chromatograms.METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃ thermostatic Thermo Acclaim TM 120 C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 240, 246 nm.RESULTS Morroniside, loganin, psoralen, isopsoralen showed good linear relationships within the ranges of 2.481 8-124.087 6, 2.328 5-116.424 0, 1.064 8-53.239 8, 1.528 3-76.416 0 μg/mL(r≥0.999 9), whose average recoveries were 102.43%, 98.61%, 92.02%, 96.93% with the RSDs of 3.75%, 5.79%, 3.46%, 5.80%, respectively. There were six characteristic peaks in the characteristic chromatograms of fifteen batches of samples with the similarities of more than 0.997-0.999.CONCLUSION This simple, reliable and reproducible method can be used for the quality control of Bushen Jiedu Granules.

关 键 词：补肾解毒颗粒 莫诺苷 马钱苷 补骨脂素 异补骨脂素 特征图谱 HPLC 

分 类 号：R927􀆰[医药卫生—药学]