高通量测序对脓毒症病原学诊断及病原菌耐药性预测的应用价值  

Application value of high-throughput sequencing in pathogenic diagnosis and drug-resistance prediction of pathogenic bacteria in sepsis

作　　者：韩林[1] 蒋玲玉[1] 黄璐 胡苗(综述) 熊滨(审校)[1] HAN Lin;JIANG Ling-yu;HUANG Lu(Department of Critical Care Medicine, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China)

机构地区：[1]广西壮族自治区人民医院重症医学科,南宁530021

出　　处：《中国临床新医学》2020年第6期642-646,共5页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

摘　　要：脓毒症是机体对感染反应失调所致的危及生命的器官功能障碍,是急危重症医学面临的重要疾病,也是临床亟待解决的难题。明确病原学后尽早针对性抗感染治疗可降低病死率,是治疗脓毒症的关键。目前的病原学检测技术在及时性和准确性等方面仍存在欠缺。高通量测序技术在诊断病原微生物方面,具有检测种类多、快速、精准等特点,且近年对于监测病原学及耐药性方面有特殊优势,并逐步应用于临床。该文就高通量测序对脓毒症病原学诊断及病原菌耐药性预测的应用价值进行综述。Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the body′s response to infections and remains a challenge in emergency and critical care medicine and also is a clinical problem to be solved urgently.Targeted anti-infection treatment as soon as possible after the pathogeny is clear can reduce the mortality,which is the key to the treatment of sepsis.At present,the detection technology of pathogeny is still lacking in timeliness and accuracy.In the diagnosis of pathogenic microorganisms,high-throughput sequencing technology can detect a wide range of species with rapid and accurate characteristics.In recent years,high-throughput sequencing has special advantages on monitoring pathogeny and drug resistance,and is being gradually applied to clinical medicine.In this paper,we review the value of high-throughput sequencing technology in pathogenic diagnosis of sepsis and prediction of drug resistance of pathogenic bacteria.

关 键 词：高通量测序 脓毒症 病原学 病原菌耐药 

分 类 号：R37[医药卫生—病原生物学]