主客体作用在生化分析中的应用研究进展  

Recent Advance in Applications of Host-Guest Interaction in Biochemical Analysis

作　　者：崔琳 赵敏惠 张春阳[1] CUI Lin;ZHAO Min-Hui;ZHANG Chun-Yang(College of Chemistry,Chemical Engineering and Materials Science,Shandong Normal University,Jinan 250014,China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《分析化学》2020年第7期817-826,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21735003,21527811,21605096,21974080);山东省泰山学者优势特色学科人才团队项目资助。

摘　　要：主客体作用是主体和客体在满足结构互补和能量匹配的条件下,通过非共价相互作用选择性结合,形成具有特定功能的超分子的过程。生物体系中的抗原-抗体、DNA-蛋白质、酶-底物等生物分子之间的识别都建立在非共价作用识别的基础上。主客体识别反应具有反应条件温和、反应过程动态可逆等特点,不仅可有效克服共价结合的局限性,而且能在分子水平上模拟生物功能。本文综述了主客体作用在生化分析中的应用的最新研究进展,并对基于主客体作用的传感器的发展趋势做了展望。Host-guest interaction is the process in which the host selectively combines the guest to form a supramolecular with specific function through non-covalent bonding according to the principles of structural complementarity and energy matching.The identification of biomolecules through antigen-antibody,DNA-protein,enzyme-substrate interactions in biological systems is based on the recognition of non-covalent interactions.The host-guest interaction possesses distinct characteristics of mild reaction conditions and dynamic reversible reaction process,and it not only can efficiently overcome the limitations of covalent bonding,but also can simulate the biological functions at the molecular level.This review summarizes the advance in the applications of host-guest interaction in biochemical analysis including the detection of proteins,nucleic acids,small biological molecules,metal ions and isomers.In addition,the challenges and trends in the development of biosensors based on host-guest interaction are highlighted.

关 键 词：主客体作用 生物传感器 超灵敏检测 生化分析 评述 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O641.3[自动化与计算机技术—控制科学与工程]