静磁场对大鼠下颌骨骨折愈合的研究  

Effect of Static Magnetic Field on Mandibular Fracture Healing in Rats

作　　者：刘阳[1] 李善昌[1] 王长振 周红梅 董国福 赵雪龙 刘琦 LIU Yang;LI Shanchang;WANG Changzhen;ZHOU Hongmei;DONG Guofu;ZHAO Xuelong;LIU Qi(Department of Affiliated Stomatological Hospital, Jiamusi University, Jiamusi 154002, China;Department of Institute of Radiation Medicine, Military Academy of Military Sciences, Beijing 100850, China)

机构地区：[1]佳木斯大学附属口腔医院,黑龙江佳木斯154002 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《口腔医学研究》2020年第6期548-553,共6页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:31800053);北京市自然科学基金(编号:5194029、4194097)。

摘　　要：目的:研究静磁场(Static Magnetic Field,SMF)对大鼠下颌骨骨折愈合过程的影响。方法:SD大鼠60只,随机分为3组:骨折手术(C组);骨折手术+骨折处覆盖陶瓷(S组);骨折手术+骨折处覆盖磁场强度为0.1T的钕磁铁(L组)。术后每周考察SD大鼠的体重、饮食、饮水;在2、4、6、8周取材。X线片检查骨折区愈合情况;ELISA检测血清中BMP-2(bone morphogenetic protein-2,BMP-2)、IL-1(Interleukin-1,IL-1)表达情况;偶氮胂Ⅲ法检测血清钙值;HE(hematoxylin-eosin staining)观察骨折区;免疫组化检测成骨相关基因BMP-2、OPG(osteoprotegerin,OPG)、OCN(osteocalcin,OCN)。结果:X线结果显示L组的骨折愈合效果最好;L组血清中的BMP-2、IL-1、钙值表达高于S、C组;HE染色观察L组新生骨小梁数量明显多于C组和S组;免疫组化结果显示静磁场可以促进BMP-2、OPG、OCN的合成和分泌。结论:静磁场可有效促进骨折的愈合。Objective:To study the effect of static magnetic field on the healing process of mandibular fracture in rats.Methods:60 SD rats were randomly divided into three groups:fracture operation(group C),fracture operation+fracture covered ceramic(group S),fracture surgery+neodymium magnet with 0.1T covering magnetic field at fracture site(Group L).The body weight,diet,and drinking water of SD rats were investigated every week after operation,and the samples were taken after 2,4,6,and 8 weeks.Bone fracture healing was observed by X-ray examination.Expression of BMP-2 and IL-1 in serum was detected by ELISA.Serum calcium was determined by arsenazoⅢmethod.Fracture area was observed by HE staining.Osteogenesis related genes BMP-2,OPG,and OCN were immunohistochemical detected.Results:X-ray results showed that group L had the best effect of fracture healing,the expressions of BMP-2,IL-1,and calcium in group L were higher than those in group S and C.HE staining showed that the number of new bone trabeculae in group L was significantly more than that in group C and group S.Immunohistochemical results showed that magnetostatic field could promote the synthesis and secretion of BMP-2,OPG,and OCN.Conclusion:Static magnetic field can effectively promote fracture healing.

关 键 词：静磁场 骨折愈合 成骨因子 

分 类 号：R68[医药卫生—骨科学]