安徽省部分地区猪贾第虫PCR检测及阳性株的分子特性  

PCR Detection and Molecular Characteristics of the Positive Strain of Giardia duodenalis in Parts of Anhui Province

作　　者：方追 贾昌泽 黄佳敏 任琦 刘欣超 顾有方[1] 李文超[1] FANG Zhui;JIA Chang-ze;HUANG Jia-min;REN Qin;LIU Xin-chao;GU You-fang;LI Wen-chao(College of Animal Science,Anhui Science and Technology University,Fengyang,Anhui,233100,China;Liuan City Center For Animal Disease Prevention And Control,Liuan,Anhui,237000,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]六安市动物疫病预防与控制中心,安徽六安237000

出　　处：《动物医学进展》2020年第6期55-58,共4页Progress In Veterinary Medicine

基　　金：安徽省自然科学基金项目(1808085MC84,1908085QC116);安徽科技学院重点学科项目(AKZDXK2015A04);安徽科技学院校级稳定人才项目(dkwd201702)。

摘　　要：为了解安徽省规模化猪场贾第虫感染情况及分子特性,从安徽省多地共采集500份新鲜猪粪样,提取基因组DNA,首先采用基于蓝氏贾第虫SSUrRNA基因套式PCR对所有粪便进行检测,并对获得的阳性样本分别基于贾第虫TPI和GDH基因的PCR扩增,然后对获得的PCR产物进行测序和分析,确定贾第虫的聚集体。结果显示,500份猪粪便样品中,SSUrRNA基因套式PCR仅在利辛猪场检测出1例贾第虫阳性样本,猪贾第虫阳性率为0.20%。TPI和GDH基因的测序分析显示该贾第虫基因型为聚集体E。To determine the prevalence and molecular characteristics of the isolates of G.duodenalis in Anhui province,500 fecal samples were detected by PCR targeting of SSUrRNA gene for the presence of G.duodenalis after fecal samples were collected and genomic DNA was extracted.Subsequently a nested-PCR targetingTPI gene and a hemi-nested-PCR targeting GDH gene were performed in the SSUrRNA-positive samples.The obtained PCR products were sequenced and bioinformatically analyzed to identify the assemblage of G.duodenalis.Of the 500 pig feces samples,only 1 positive sample of G.duodenalis from the pig farm in Lixin was detected by nested PCR of SSUrRNA gene,and the positive rate of G.duodenalis in pigs in Anhui province was 0.20%.Sequencing analysis of TPI and GDH genes showed that the genotype of the isolate obtained in this study was assemblage E.

关 键 词：蓝氏贾第虫 小亚基核糖体rRNA基因 磷酸丙糖异构酶 谷氨酸脱氢酶 猪 

分 类 号：S852.739[农业科学—基础兽医学]