基于内病外治法研制的筋骨痛巴布膏质量标准研究  

Quality standard for cataplasma for bone and muscle pain developed by the method of external treatment for internal diseases

作　　者：赵启[1] 张琼 胥骞 胥双 袁清照[1] ZHAO Qi;ZHANG Qiong;XU Qian;XU Shuang;YUAN Qingzhao(The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China;Xiangtan Medicine&Health Vocational College,Xiangtan 411104,Hunan,China)

机构地区：[1]湖南省中医药研究院附属医院,湖南长沙410006 [2]湘潭医卫职业技术学院,湖南湘潭411104

出　　处：《湖南中医杂志》2020年第5期148-150,154,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药科研计划项目(201766)。

摘　　要：目的:建立筋骨痛巴布膏的质量标准。方法:筛选方中独活、大黄、苏木及冰片进行薄层色谱鉴别;HPLC法对成品中大黄素进行含量测定。结果:制定了巴布膏中独活等药材的薄层鉴别方法;以大黄素为指标,以Kromasil C18色谱柱(250 mm×4. 6 mm,5μm)为固定相,甲醇-0. 1%磷酸溶液(85∶15)为流动相;检测波长254 nm条件下,成品中大黄素能有效分离,方法学结果可行。结论:该法方便准确、灵敏度高、重现性好,能够为筋骨痛巴布膏质量标准的制订提供客观精准的依据。Objective: To establish the quality standard for cataplasma for bone and muscle pain. Methods: Thin-layer chromatography( TLC) was performed for Angelica pubescens,Rheum officinale,hematoxylon,and borneol in the prescription,and high-performance liquid chromatography( HPLC) was used to determine the content of emodin in the finished product. Results: The TLC identification method was established for the medicinal materials,including Angelica pubescens,in the cataplasma. With the content of emodin as an index,HPLC was performed on a Kromasil C18 column( 250 mm × 4. 6 mm,5 μm) with a mobile phase of methanol-0. 1% phosphoric acid solution( 85∶ 15),and at a detection wavelength of 254 nm,emodin was separated effectively from the finished product. The results of methodology showed that this method was feasible. Conclusion: This method is convenient and accurate,with high sensitivity and good repeatability,and therefore,it can provide objective and accurate evidence for developing the quality standard for cataplasma for bone and muscle pain.

关 键 词：内病外治 筋骨痛巴布膏 质量标准 薄层色谱法 

分 类 号：R284[医药卫生—中药学]