三种皮肤修复材料对糖尿病大鼠皮肤损伤修复的有效性评价  

Evaluation of Three Kinds of Skin Repair Materials on the Effectiveness of Skin Injury Repair in Diabetic Rats

作　　者：韩倩倩[1] 曲明悦 薛彬 王春仁[1] HAN Qianqian;QU Mingyue;XUE Bin;WANG Chunren(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京市102629

出　　处：《组织工程与重建外科杂志》2020年第3期232-236,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：科技部十三五重点研发计划(2016YFC1103202);中国科学院“器官重建与制造”战略性先导专项(XDA16040602)。

摘　　要：目的评价胶原蛋白膜、羧甲基壳聚糖止血海绵和明胶生物活性玻璃复合材料对于修复糖尿病大鼠背部皮肤损伤的有效性。方法SD雄性大鼠腹腔单次注射不同浓度的STZ溶液,并在背部切除不同大小的全层皮肤,以期建立最佳的糖尿病大鼠皮肤损伤模型。三种皮肤修复材料直接贴在损伤处,并用无菌敷料贴覆盖,观察这三种材料对创面恢复情况和伤口愈合速率的影响。结果单次腹腔注射75 mg/Kg的STZ溶液、构建2 cm×2 cm的背部皮肤损伤可建立最佳糖尿病大鼠皮肤损伤模型。胶原和明胶生物玻璃均有一定程度促进该模型皮肤愈合的作用,愈合率与空白对照相比有统计学差异。结论胶原和明胶生物玻璃对糖尿病大鼠的皮肤损伤有治疗作用,羧甲基壳聚糖仅在早期发挥止血作用。Objective To evaluate the effectiveness of collagen,carboxymethyl chitosan and gelatin bioactive glass in repairing the skin injury of diabetic rats.Methods The best skin injury model of diabetic rats was established by injecting different concentration of STZ solution in single time and excising the whole skin of different sizes in the back of SD male rats respectively.Three kinds of skin dressings were attached directly to the injured area and covered with sterile dressings in order to promote skin healing.The general wound recovery was observed and wound healing rate of each group was calculated.HE staining was used to observe the recovery of wound in different periods.Results A single intraperitoneal injection of 75 mg/Kg STZ solution and the construction of 2 cm×2 cm back skin injury could establish the best skin injury model of diabetic rats.Collagen and gelatin bioactive glass promoted skin healing to a certain extent,and the healing rate was statistically different from the blank control.Conclusion Collagen and gelatin bioactive glass have therapeutic effect on skin injury of diabetic rats,carboxymethyl chitosan only has hemostatic effect in early stage.

关 键 词：糖尿病 皮肤损伤 胶原蛋白 羧甲基壳聚糖 明胶生物玻璃 

分 类 号：R628[医药卫生—整形外科]