慢性乙型肝炎不同临床分型患者IL-12水平及Th1/Th2细胞平衡的相关性研究  

Correlation between IL-12 level and Th1/Th2 cell balance of patients with different clinical types of chronic hepatitis B

作　　者：侯丽 赖辉[1] 何兴[1] 胥菁雯 江珊 HOU Li;LAI Hui;HE Xing;XU Qing-wen;JIANG Shan(Guangyuan First People′s Hospital,Guangyuan,Sichuan 628017,China)

机构地区：[1]广元市第一人民医院感染科,四川广元628017

出　　处：《中华医院感染学杂志》2020年第9期1311-1314,共4页Chinese Journal of Nosocomiology

基　　金：四川省科研基金资助项目(2018252)。

摘　　要：目的探究慢性乙型肝炎(Chronic hepatitis B,CHB)不同临床分型患者IL-12水平与Th1/Th2细胞平衡的相关性。方法选择2018年1月-2019年4月广元市第一人民医院感染科收治的慢性乙型肝炎患者70例。根据临床分型的不同,分为轻度32例(CHBⅠ级组)、中度29例(CHBⅡ级组)和重度9例(CHBⅢ级组),选择同期体检健康者30名作为对照组。采用RT-PCR法检测血清乙型肝炎病毒(HBV)DNA含量;ELISA检测血清白细胞介素-12(Interleukin-12、IL-12)水平和HBV标志物[乙肝e抗原(HBeAg)、乙肝表面抗原(HBsAg)、乙肝e抗体(抗-HBe)]水平;检测外周血Th1、Th2及Th1/Th2细胞比例。结果 CHBⅠ级组患者血清HBV DNA病毒载量显著低于CHBⅡ级组和CHBⅢ级组(P<0.05);CHBⅠ级组HBeAg、HBsAg水平显著低于CHBⅡ级组和CHBⅢ级组,而抗-HBe水平显著高于CHBⅡ级组和CHBⅢ级组(P<0.05);CHB患者血清IL-12水平显著高于对照组(P<0.05),且随着CHB患者病情的加重,血清IL-12水平显著升高(P<0.05);CHB患者的Th2细胞比例显著高于对照组,而Th1、Th1/Th2显著低于对照组(P<0.05),随着CHB患者病情的加重,Th2细胞比例显著升高,而Th1、Th1/Th2显著降低(P<0.05)。结论慢性乙型肝炎患者体内存在Th1/Th2失衡现象,患者病情严重程度与体内HBV DNA载量呈正比,病毒复制水平升高可能与Th1细胞减少,机体的病毒清除能力下降,特异性细胞免疫应答减弱有关。OBJECTIVE To explore the correlation between the IL-12 level and the Th1/Th2 cell balance of patients with different clinical types of chronic hepatitis B(CHB).METHODS A total of 70 patients with CHB who were treated in Guangyuan First People′s Hospital from Jan 2018 to Apr 2019 were enrolled in the study and divided into the CHB I group with 32 mild cases,the CHBⅡgroup with 29 moderate cases and the CHBⅢgroup with 9 severe cases according to the clinical classification,meanwhile,30 healthy people who received physical examination were set as the control group.The serum hepatitis B virus(HBV)DNA load was determined by using RT-PCR,the levels of serum interleukin-12(IL-12)and HBV markers[hepatitis B e Antigen(HBeAg),hepatitis B surface Antigen(HBsAg),hepatitis B e Antibody(anti-HBe)]were detected by using ELISA,and the percentages of peripheral blood Th1 and Th2 as well as Th1/Th2 were detected.RESULTS The serum HBV DNA load of the CHBⅠgroup was significantly lower than that of the CHBⅡgroup and the CHBⅢgroup(P<0.05).The levels of HBeAg and HBsAg of the CHBⅠgroup were significantly lower than those of the CHBⅡgroup and the CHBⅢgroup,while the anti-HBe level of the CHBⅠgroup was significantly higher than that of the CHBⅡgroup and the CHBⅢgroup(P<0.05).The serum IL-12 level of the patients with CHB was significantly higher than that of the study objects in the control group(P<0.05),and the serum IL-12 level was significantly elevated with the exacerbation of illness condition of the CHB patients(P<0.05).The percentage of Th2 cell of the CHB patients was significantly higher than that of the study objects in the control group,while the percentage of Th1 cell and Th1/Th2 were significantly lower in the CHB patients than in the study objects in the control group;with the exacerbation of illness condition,the percentage of Th2 cell of the CHB patients was significantly elevated,while the percentage of Th1 cell and Th1/Th2 were significantly reduced(P<0.05).CONCLUSION There is a Th1/Th2 imbalance

关 键 词：慢性乙型肝炎 临床分型 HBV DNA IL-12 TH1/TH2 

分 类 号：R512.62[医药卫生—内科学]