离轴抛物镜扫描中继系统提升双光子显微成像视场  

Off-axis Parabolic Mirror Afocal Scanning System Extends the Imaging Area of Two-photon Microscopy

作　　者：姚靖 吴婷 叶世蔚 高玉峰[3] 郑炜[3] 秦水介 YAO Jing;WU Ting;YE Shiwei;GAO Yufeng;ZHENG Wei;QIN Shuijie(Guizhou University Department of Physics,Guiyang 550025,China;Guizhou University,Key Laboratory of Optoelectronic Technology and Application of Guizhou,Guiyang 550025,China;Research Laboratory for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China)

机构地区：[1]贵州大学物理学院,贵阳550025 [2]贵州大学贵州省光电子技术与应用重点实验室,贵阳550025 [3]中国科学院深圳先进技术研究院生物医学光学与分子影像研究室,深圳518055

出　　处：《激光生物学报》2020年第3期217-224,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金仪器项目(81927803);国家自然科学基金优秀青年科学基金项目(82822023);深圳市基础研究学科布局项目(JCYJ20170818164343304)。

摘　　要：本文提出利用离轴抛物镜共焦中继系统来改善双光子成像视场边缘像质变差的问题,进而提升双光子成像视场。不同于传统的双胶合透镜扫描中继系统,离轴抛物镜共焦扫描中继系统由一对离轴抛物镜构成,扫描振镜位于抛物面镜焦点处。仿真和试验结果均显示,该系统能有效优化视场边缘的像散、场曲和畸变情况,提高成像质量。利用该扫描系统,我们实现了视场为2.4 mm×2.4 mm,横向分辨率为1μm的大视场双光子显微成像,能清晰分辨小鼠大脑切片中的神经轴突结构。We propose an off-axis parabolic mirror(OAPM)afocal scanning system which can improve the image quality in the marginal area of two-photon microscopy.Compared with the traditional double lenses afocal scanning system,the OAPM afocal scanning system consists of a pair of parabolic mirrors,and a galvo scanner is located at one of the foci of the parabolic mirror.Both the simulation and experiment results show that the OAPM afocal scanning system can significantly reduce the astigmatism,the field curvature and the distortion introduced on the edge of field of view.Furthermore,adopting the proposed afocal scanning system,we build a two-photon microscope with a field of view of 2.4 mm×2.4 mm and a lateral resolution of 1μm.Using this system,the neuron axons in the mouse brain slice can be clearly resolved.

关 键 词：离轴抛物面反射镜 双光子成像 共焦扫描中继 像差 大视场 

分 类 号：O435[机械工程—光学工程]