3D打印β-磷酸三钙仿生骨支架修复兔股骨髁骨缺损  

Experimental study on biomimetic bone scaffold of beta-tricalcium phosphate prepared by 3D printing technology in repairing bone defects

作　　者：段钢 陈宏亮 郭开今 陈向阳 Duan Gang;Chen Hongliang;Guo Kaijin;Chen Xiangyang(Department of Orthopedics, Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China)

机构地区：[1]徐州医科大学第二附属医院骨科 [2]徐州医科大学附属医院骨科,221000

出　　处：《骨科临床与研究杂志》2020年第4期243-250,共8页Journal Of Clinical Orthopedics And Research

基　　金：徐州市科学技术局重点研发计划(KC17197);中国博士后科学基金第62批面上资助项目(2017M621826);江苏省博士后科学基金C类项目。

摘　　要：目的研究3D打印β-磷酸三钙(β-TCP)仿生骨支架的生物学与生物力学性能及其在骨缺损修复中的作用。方法应用3D打印技术针对实验兔股骨髁制备个性化β-TCP仿生骨支架25枚,采用扫描电子显微镜观察支架的结构形态并计算孔隙率,采用万能力学试验机体外测定支架的生物力学强度。选取实验兔30只,随机分为实验组(15只)和对照组(15只),行手术制作兔股骨髁骨缺损模型。对实验组兔股骨髁骨缺损植入β-TCP仿生骨支架进行修复,对对照组兔股骨髁骨缺损不植入修复材料。在术后第4、8、12周分别取两组各5只兔在麻醉下行手术,对实验组兔植入支架部位和对照组兔骨缺损部位进行取材,以光学显微镜观察取材组织有无炎性反应、骨组织长入以及材料降解情况,并采用Lane-Sandhu组织学评分方法对骨愈合情况进行评价。采用SPSS 15.0软件对数据进行处理并对两组数据进行比较。结果β-TCP仿生骨支架的形态和结构与设计目标相符,内部结构与兔股骨相似,存在小梁和空隙且相互贯通相连,较大孔孔径为(223.02±18.20)μm,微孔孔径为(15.06±0.09)μm,孔隙率为(65.10±1.38)%,抗压强度为(3.83±0.65)MPa。实验组兔股骨髁骨缺损在术后2周完全愈合。术后第4、8、12周实验组和对照组兔股骨髁缺损部位的Lane-Sandhu组织学评分分别为(1.3±0.5)和(5.2±0.3)分,(1.8±0.4)和(5.6±0.8)分,(2.1±0.6)和(8.1±1.2)分,差异均有统计学意义(P=0.027,0.007,0.002)。结论3D打印β-TCP仿生骨支架是一种较为理想的骨缺损修复材料。Objective To study the biological and biomechanical properties of 3D printedβ-tricalcium phosphate(β-TCP)bionic bone scaffold and its role in repairing bone defects.Methods Twenty-five personalizedβ-TCP bionic bone scaffolds were prepared for femoral condyles of experimental rabbits by 3D printing technology.The structure and morphology of the scaffolds were observed by scanning electron microscope and porosity was calculated.The biomechanical strength of the scaffolds was measured in vitro by universal mechanical testing machine.Thirty experimental white rabbits were selected and randomly divided into the experimental group(15 rabbits)and the control group(15 rabbits).The rabbit femoral condyle defect model was made by surgery.Beta-TCP bionic bone scaffold was implanted to repair the femoral condylar bone defect in the experimental group,while no repair material was implanted to the femoral condylar bone defect in the control group.At 4th,8th,and 12th week after surgery,2 groups of 5 rabbits were taken under anesthesia for surgery.The stent site in rabbits of experimental group and bone defect site in rabbits of control group were taken for sampling.The inflammatory reaction,bone tissue ingrowth and material degradation of the selected tissues were observed by optical microscope,and the bone healing was evaluated by Lane-Sandhu histological scoring method.SPSS 15.0 software was used to process the data and compare the 2 groups of data.Results The morphology and structure of theβ-TCP bionic bone scaffold were consistent with the design objectives.The internal structure of the scaffold was similar to that of rabbit femur,with trabeculae and voids connected with each other.The larger pore diameter was(223.02±8.20)μm,the micropore diameter was(15.06±0.09)μm,the porosity was(65.10±1.38)%,and the compressive strength was(3.83±0.65)MPa.The femoral condyle defect in the experimental group healed completely 2 weeks after operation.At the 4th,8th and 12th weeks after operation,the Lane-Sandhu histological scores

关 键 词：磷酸钙类 组织支架 打印 三维 骨和骨组织 创伤和损伤 骨再生 模型 动物 兔 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]