两种检测系统检测血清淀粉样蛋白A的结果比较  

Effect comparison of two methods in the detection of serum amyloid A protein

作　　者：孙丽 张诗海[2] SUN Li;ZHANG Shi-hai(Department of Clinical Laboratory,The Third People′s Hospital of Hefei,Hefei Anhui 230038;Department of Clinical Laboratory,Anhui Provincial Children′s Hospital,Hefei Anhui 230051,China)

机构地区：[1]安徽省合肥市第三人民医院检验科,230038 [2]安徽省儿童医院检验科,安徽合肥230051

出　　处：《蚌埠医学院学报》2020年第6期812-814,共3页Journal of Bengbu Medical College

摘　　要：目的:比较免疫速率散射比浊法和免疫荧光层析法检测血清淀粉样蛋白A(serum amyloid A protein,SAA)的结果。方法:收集住院病人(观察组)和健康体检者(对照组)血清,均分别采用全自动特定蛋白检测仪(采用免疫速率散射比浊法)和荧光免疫定量分析仪(免疫荧光层析法)检测SAA水平,比较2种检测系统上的分析结果。结果:观察组血清用2种方法检测的SAA水平均高于对照组(P<0.01);观察组免疫速率散射比浊法所测SAA值高于免疫荧光层析法(P<0.01),而对照组采用2种方法所测SAA值差异无统计学意义(P>0.05)。免疫速率散射比浊法和免疫荧光层析法检测的敏感度分别为53.00%和57.00%,特异度分别为92.00%和93.00%,差异均无统计学意义(P>0.05)。荧光免疫层析法对健康体检者所测SAA的下限概率为83.00%(83/100),高于免疫速率散射比浊法的42.00%(42/100)(χ^2=35.86,P<0.01),荧光免疫层析法检测低值时候更稳定。结论:免疫速率散射比浊法和免疫荧光层析均能用于临床检测,后者的稳定性更好。Objective:To compare the effects between immunovelocity nephelometry and immunofluorescence chromatography in the detection of serum amyloid protein A(SAA).Methods:The serum levels of SAA of inhospital patients(observation group)and healthy physical examinees(control group)were detected using the full-automatic specific protein detector(immunovelocity nephelometry)and fluorescence immunoquantitative analyzer(immunofluorescence chromatography),and the detection results were compared between two methods.Results:The serum levels of SAA deetcted by two methods in observation grop were higher than those in control group(P<0.01).The serum level of SAA in observation group detected by immunovelocity nephelometry was higher than that of immunofluorescence chromatography(P<0.01),while the difference of the serum level of SAA in control group was not statistically significant between two detection methods(P>0.05).The sensitivities and specificities of immunovelocity nephelometry and immunofluorescence chromatography were 53.00%&57.00%and 92.00%&93.00%,respectively,and the differences of those were not statistically significant between two methods(P>0.05).The lower limit probability of SAA detected by fluorescence immunochromatography in control group(83.00%,83/100)was higher that of immunovelocity nephelometry(42.00%,42/100)(χ^2=35.86,P<0.01).The fluorescence immunochromatography in the detection of low value was more stable.Conclusions:The immunovelocity nephelometry and immunofluorescence chromatography can be used for clinical detection,the latter has better stability.

关 键 词：免疫速率散射比浊法 免疫荧光层析法 血清淀粉样蛋白A 

分 类 号：R446[医药卫生—诊断学]