虫草素对大鼠关节炎及TNF-α诱导的FLS细胞的影响  

Effect and Mechanism of Cordycepin in Rat ACLT Model and TNF-α-induced Fibroblast-like Synoviocytes

作　　者：吴辉[1] 肖东琴[1] 郭明岗 李亮 罗旭伟 WU Hui;XIAO Dongqin;GUO Minggang;LI Lang;LUO Xuwei(Department of Orthopedics,Nanchong Central Hospital,The Second Clinical Medical College of North Sichuan Medical College,Nanchong,Sichuan,637000,China)

机构地区：[1]川北医学院第二临床医学院南充市中心医院骨科,四川省南充市637000

出　　处：《医学分子生物学杂志》2020年第2期115-120,128,共7页Journal of Medical Molecular Biology

基　　金：四川省科学技术厅科技计划项目(No.2016JY0123)。

摘　　要：目的探究虫草素(Cordycepin,CDP)对膝前交叉韧带切断手术(transection of anterior cruciate ligament,ACLT)模型大鼠及大鼠成纤维样滑膜细胞的作用及机制。方法ACLT方法建立大鼠骨关节炎(osteoarthritis,OA)模型,原代成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)进行肿瘤坏死因子α(tumor necrosis factorα,TNF-α)(10 ng/ml)诱导30 min;CDP处理大鼠7 d。HE、Masson染色观察细胞形态及纤维化,Western印迹鉴定分泌蛋白及凋亡分子。ELISA检测血清中炎性因子。Hoechst染色鉴定细胞凋亡。结果在体内实验中,与Ctrl组比较,CDP改善软骨细胞的形态和纤维化状况,增加Ⅱ型胶原蛋白(collagenⅡ,COⅡ)和聚蛋白聚糖(aggrecan)的分泌(P<0.01);同时降低TNF-α、白细胞介素6(interleukin-6,IL-6)、基质金属蛋白酶3(matrix metalloproteinase,MMP-3)的表达(P<0.01)。CDP可以降低FLS细胞增殖,并促进受损细胞凋亡(P<0.01)。结论CDP通过降低TNF-α、IL-6、MMP-3的表达,降低大鼠软骨细胞的炎症和生长,促进受损细胞凋亡。Objective The aim of the study was to investigate the effect and mechanism of cordycepin(CDP)on rats with transection of anterior cruciate ligament(ACLT)and TNF-α-in-duced fibroblast-like synoviocytes.Methods For this purpose,HE/MASSON staining was per-formed to investigate the cell morphology and chondrocytes fibrosis.The inflammatory-and apoptosis-and fibrotic-related protein levels were determined by Western blotting.The levels of inflammatory factors were detected by ELISA.Cells apoptosis was detected by Hoechst staining.Results CDP im-proved the morphology and fibrosis of chondrocytes and enhanced the expression of CollagenⅡ(COⅡ)and Aggrecan,and decreased the TNF-α,IL-6 and MMP-3(P<0.01).CDP inhibited the cell growth and promoted the cell apoptosis(P<0.01).Conclusion CDP can inhibit the inflam-mation and cell growth rate while promote the cell apoptosis,that may be associated with the activa-tion of TNF-αand its downstream pathway.

关 键 词：虫草素 骨关节炎 成纤维滑膜细胞 炎性因子 凋亡 细胞增殖 

分 类 号：R684.3[医药卫生—骨科学]