Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge  

作　　者：Woong Jin Bae Jin Bong Choi Kang Sup Kim U Syn Ha Sung Hoo Hong Ji Youl Lee Tae-Kon Hwang Sung Yeoun Hwang WANG Zhi-ping Sae Woong Kim 

机构地区：[1]Department of Urology,The Catholic University of Korea,College of Medicine,Seoul(06591),Republic of Korea [2]Catholic Integrative Medicine Research Institute,College of Medicine,The Catholic University of Korea,Seoul(06591),Republic of Korea [3]Korea Bio-Medical Science Institute,Seoul(06106),Republic of Korea [4]Department of Urology,Second Hospital of Lanzhou University,Lanzhou(730030),China

出　　处：《Chinese Journal of Integrative Medicine》2020年第7期533-538,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Bio&Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science&ICT(No.2018M3A9E8020861),Republic of Korea。

摘　　要：Objective:To investigate the antiproliferative activity of Salvia miltiorrhiza Bunge.(SM)on the castration-resistant prostate cancer(CRPC)cell line DU-145 in vitro and in vivo.Methods:Prostate cancer cell line(DU-145)and normal prostate cell line(RWPE-1)were treated with SM at different concentrations(3.125,12.5,25 and 50μg/mL)to investigate the antiproliferative effects.DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells.Molecular mechanism was investigated by Western blot analysis of p53,Bcl-2,prostate specific antigen(PSA),and androgen receptor(AR).Six-week-old male BALB/c nude mice were randomly divided into normal control group(n=101)and treated group(n=101)which administered 500 mg/kg SM for 2 weeks.Tumor volumes were measured.Results:Treatment with SM resulted in a dose-dependent decrease in cell number of DU-145 cells in comparison with RWPE-1.DNA laddering analysis indicated the apoptosis of DU-145 cells.Treatment with SM increased the expression of p53 and reduced the expression of Bcl-2 proteins.The levels of PSA were considerably reduced in SM-treated group compared to the controls,and a decrease in AR expression was observed when cells were treated with SM in the same pattern as a reduction in PSA.In the tumour xenograft study,SM given once a day for 2 weeks significantly inhibited tumour growth.Conclusion:SM might contribute to the anticancer actions such as induction of apoptosis and inhibition of proliferation of prostate cancer cells.

关 键 词：apoptosis prostate cancer Salvia miltiorrhiza Bunge Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]