过表达OsPGIP基因对甘蓝型油菜生长发育影响  

The effects of overexpression of OsPGIP gene on the Brassica napus growth and development

作　　者：李师 李国林 王睿 赵云[1] LI Shi;LI Guo-Lin;WANG Rui;ZHAO Yun(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China)

机构地区：[1]四川大学生命科学学院,生物资源与环境教育部重点实验室,四川成都610065

出　　处：《四川大学学报（自然科学版）》2020年第4期811-817,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技厅项目(2017SZ0181,2018NZDZX0003);国家重点研发计划(2018YFC1802605)。

摘　　要：本实验旨在探究在油菜中过表达OsPGIP基因对其生长发育的影响.实验包括种子品质指标及幼苗生长状况测定,利用Nikon SMZ18荧光体式显微镜观察花器官形态,RT-PCR鉴定OsPGIP基因表达量,POD,SOD,CAT等抗氧化酶酶活测定,角果数、有效分枝数及初花天数等重要农艺性状统计.结果显示:与野生型相比,各转基因家系T1代种子千粒重、发芽率及幼苗生长无明显缺陷,柱头及花药器官发育正常,初花天数缩短约8天.本文研究发现过表达PGIP基因能够显著缩短油菜的初花天数,但对其正常生长、结实无明显影响.In this experiment,it was designed to investigate the effects of overexpression of OsPGIP gene on the Brassica napus growth and development.The seed quality index and seeding growth condition were determined in this study.We also observed the flower organ morphology by Nikon SMZ18 fluorescence microscope and detected OsPGIP gene expression level by RT-PCR.Besides,this experiment also includes morphological observation of flower organs,determination activities of antioxidant enzymes such as POD,SOD,CAT,etc.,and statistics of important agronomic characters such as the number of pods,effective branches and days of first flowering.The results showed that there were no obvious defects in seed quality and seedling growth of T1 generation transgenic families,the development of flower organs was normal and the days of first flowering were shortened about 8 days compared with the wild type.Our data indicate that overexpression of PGIP gene could significantly shorten the first flowering days of rape,but had no significant effect on its normal growth and seed setting.

关 键 词：PGIP基因 抗氧化酶 千粒重 角果数 

分 类 号：Q945[生物学—植物学]