反相微乳法制备羟基磷灰石纳米晶体  

Preparation of Hydroxyapatite Nanocrystal by Reverse Microemulsion Method

作　　者：毛春玲 刘雅茹 吴孟霏 孙玉琦 MAO Chun-ling;LIU Ya-ru;WU Meng-fei;SUN Yu-qi(Colloge of Pharmacy,Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]锦州医科大学药学院,辽宁锦州121001

出　　处：《化学试剂》2020年第6期699-703,共5页Chemical Reagents

基　　金：国家级大学生创新创业训练计划项目(2019101-60063)。

摘　　要：采用反相微乳法制备羟基磷灰石纳米晶体,以正己烷为有机相、水为水相、CTAB与正己醇为乳化剂和助乳化剂,通过三元体系三相图优化各组分的比例。CaCl2与Na2HPO4为反应物,通过电导率确定反应物的浓度。并对所制备样品的钙含量、制剂学性质和体外释放行为进行考察。结果表明采用反相微乳法制备羟基磷灰石纳米晶体工艺可行,m(有机相)∶m(乳化剂相)=1∶1,m(CTAB)∶m(正己醇)=1∶2。0.12 mol/L的Na2HPO4与同体积0.2 mol/L的CaCl2反应,所制备样品为羟基磷灰石纳米晶体,样品粒径较小,并具有较好的制剂学性质和体外释放行为。n-Hexane(organic phase),CTAB(emulsifier),n-hexanol(co-emulsifier)and CaCl2,Na2HPO4(reactants)were used to prepare hydroxyapatite nanocrystal by a reverse microemulsion method.The three-phase diagrams of ternary system and conductivity were used to optimize the composition and concentration.The calcium contents,pharmaceutical properties and release behavior of the samples in vitro had been investigated.The results showed that it was feasible to prepare hydroxyapatite nanocrystal by a reverse microemlsion method.The mass ratio of organics-emulsifier phase and CTAB-n-hexanol was 1∶1 and 1∶2,respectively.Na2HPO4(0.12 mol/L)reacted with equal volumes of CaCl2(0.2 mol/L)to generate hydroxyapatite.The sample was confirmed as hydroxyapatite nanocrystal.It had better pharmaceutical properties and release behavior in vitro with smaller particle size.

关 键 词：羟基磷灰石纳米晶体 反相微乳法 三相图 电导率 制备与表征 

分 类 号：R944[医药卫生—药剂学]