生物合成槲皮素糖苷类衍生物的研究进展  

Advances in biosynthesis of quercetin glycoside derivatives

作　　者：封悦洋 王颖[2,3] 姚明东 肖文海[2,3] 丁明珠[2,3] FENG Yueyang;WANG Ying;YAO Mingdong;XIAO Wenhai;DING Mingzhu(Frontier Technology Research Institute,Tianjin University,Tianjin 301700,China;Frontier Science Center for Synthetic Biology,Tianjin University,Tianjin 300072,China;Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学前沿技术研究院,天津301700 [2]天津大学合成生物学前沿科学中心,天津300072 [3]天津大学系统生物工程教育部重点实验室,天津300072

出　　处：《化工学报》2020年第7期2945-2955,共11页CIESC Journal

基　　金：国家重点研发计划项目(2018YFA0900702);天津市自然科学基金一般项目(17JCYBJC23900)。

摘　　要：糖苷类天然产物是植物中次级代谢产物的主要存在形式,具有重要的生物活性。通过糖基化修饰可以改变其水溶性、稳定性,产生特殊的生物活性和功能,因此易于商品化应用,具有重要的药用价值和工业价值,尤其在抗癌药物和日化用品中有着广泛应用。近年来糖苷类产物的生物合成也取得了重大的进展。以重要的糖苷化合物槲皮素为例介绍了糖苷化合物的生物合成,从糖基转移酶和前体供应等角度阐述了生物高产糖苷类化合物的工程策略,为利用合成生物学技术获得植物糖苷的高产菌株提供了技术参考。Natural products of glycosides are the main forms of secondary metabolites in plants and have important biological activities.Glycosylation modification can change its water solubility and stability,and produce special biological activities and functions.Therefore,glycoside products are suitable for commercialization and have important medicinal and industrial values.Especially it is widely applied to anti-cancer drugs and daily chemical products,thus glycoside compounds have been concerned and studied.In recent years,the biosynthesis of glycoside products had made great progress.In this paper,the important glycoside compound quercetin is illustrated to introduce the biosynthesis of glycoside compounds,and the engineering strategy of high-yield glycoside compounds is described from the perspective of glycosyltransferase and precursor supply.It provides technological support for researchers to obtain the high-yield strains of plant glycosides by utilizing synthetic biology techniques.

关 键 词：植物糖苷 槲皮素 UDP-糖 代谢 分子生物学 酶 

分 类 号：Q74[生物学—分子生物学]