高效液相色谱法测定活血凝胶中川续断皂苷Ⅵ含量  

Determination of Asperosaponin Ⅵ in Huoxue Gel by High Performance Liquid Chromatography

作　　者：漆伟[1] 敖珊[1] 王嫣然 任运强 常瑞[1] 周木子 刘才英[1] Qi Wei;Ao San;Wang Yanran;Ren Yunqiang;Chang Rui;Zhou Muzi;Liu Caiying(Chongqing orthopaedic hospital of Traditional Chinese Medicine,Chongqing,400012,China;Chengdu Shenghong Biotechnology Co.,Ltd.,Chengdu,611130,China)

机构地区：[1]重庆市中医骨科医院,重庆400012 [2]成都圣洪生物科技有限公司,四川成都611130

出　　处：《成都中医药大学学报》2020年第2期37-41,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：重庆市卫生与计划生育委员会中医药科技项目(ZY201702034)。

摘　　要：目的:建立活血凝胶剂中川续断皂苷Ⅵ的含量测定方法,为其质量控制提供依据。方法:采用高效液相色谱法进行含量测定。色谱柱为Aglient ZORBAX C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.25%磷酸溶液(25∶75),流速为1.0 mL/min,检测波长为212 nm。结果:川续断皂苷Ⅵ在0.37μg^1.12μg范围内线性关系良好,精密度、稳定性符合要求,阴性无干扰,平均加样回收率为96.44%,RSD为1.14%。结论:本研究所建立的川续断皂苷Ⅵ含量测定方法准确、灵敏、重复性好,可以作为评价活血凝胶的质量标准之一。Objective: To establish a determination method of Asperosaponin Ⅵ in Huoxue Gel, and to provide the basis to set up quality control standard for the product. Methods: A high performance liquid chromatography(HPLC) method was established using Aglient ZORBAX C18 column(150 mm×4.6 mm,5 μm), with acetonitrile-0.25% phosphoric acid solution(25∶75) as a mobile phase and flow rate 1.0 mL/min;detection wave length was 212 nm. Results: The calibration curve of Asperosaponin Ⅵ was in good linearity within the range of 0.37-1.12 μg;the average recovery was 96.44%, with RSD% of 1.14%. Conclusion: The established method is accurate, highly sensitive and well reproducible, which can be used for the determination of asperosaponin Ⅵ inHuoxue Gel and the control of product quality.

关 键 词：活血凝胶 含量测定 高效液相色谱法 川续断皂苷Ⅵ 

分 类 号：R284[医药卫生—中药学] R289.5[医药卫生—中医学]