抗PVY云烟87定向改良新品种“云烟301”的选育及特征特性  

Breeding and characteristic of PVY resistant new variety Yunyan301 obtained by directional modification on basis of of Yunyan87 as femal parent

作　　者：刘勇[1] 黄昌军[1] 曾建敏[1] 肖炳光[1] 于海芹[1] 李永平[1] LIU Yong;HUANG Changjun;ZENG Jianmin;XIAO Binggang;YU Haiqin;LI Yongping(Yunnan Academy of Tobacco Agricultural Sciences,National Tobacco Genetic Engineering Research Center,Kunming 650021,China)

机构地区：[1]云南省烟草农业科学研究院/国家烟草基因工程研究中心,昆明650021

出　　处：《中国烟草学报》2020年第3期59-65,共7页Acta Tabacaria Sinica

基　　金：中国烟草总公司云南省公司科技项目(2017YN02);国家自然科学基金地区科学基金项目(31860490)。

摘　　要：抗PVY云烟87新品种"云烟301"是云南省烟草农业科学研究院、国家烟草基因工程中心在克隆烟草隐性抗PVY基因eif4e1(又称为感PVY基因eIF4E1)、开发功能性分子标记、分析国内外抗PVY品种和种质资源基因型过程中,以云烟87为母本、Y85×RY2/F2为父本(携带eif4e1)杂交,以定向改良云烟87的PVY抗性为主要目标,通过连续回交、分子标记辅助选择、全基因组基因芯片背景检测等技术培育而成(非转基因)。云烟301的遗传背景恢复为云烟87的比率为99.43%,云烟301的PVY抗性显著提高,保留了云烟87的栽培烘烤特性、原烟风格特征和感官质量。在打顶后PVY发病率达到1%的云烟87种植区,种植云烟301可挽回病害损失,具有良好的推广价值。The PVY resistant“Yunyan301”is bred by the Yunnan Acedemy of Tobacco Agricultural Sciences,the National Tobacco Genetic Engineering Research Center.After cloning tobacco recessive PVY resistant gene eif4e1(also known as the PVY susceptiable gene eIF4E1),developing functional molecular markers,and analyzing the domestic and foreign PVY resistant varieties and germplasma resources genotype,through continuous backcross,molecular marker-assisted selection Yunyan301 was bred by with Yunyan87 as the female parent and Y85 x RY2/F2 as male parent(carrying eif4e1)using whole genomic chip background detection(non-GMO).The genetic background of The Genetic background recovery rate from Yunyan301 to Yunyan87 was about 99.43%.Yunyan301 has significantly improved PVY resistance compared with Yunyan87,and retains the same cultivation and curing characteristics,original leaf smoke style characteristics and sensory quality as that of Yunyan87.In the Yunyan87 planting area where the PVY incidence rate reached 1%after topping,planting Yunyan301 can recover PVY-induced loss,which is a good choice withgood prospect of promotion.

关 键 词：烤烟 PVY抗性 云烟87 基因组定向改良 

分 类 号：S572[农业科学—烟草工业]