小果卫矛愈伤组织诱导及植株再生体系的建立  

Callus induction and Plant Regeneration of Euonymus microcarpus(Oliv.)Spraggue

作　　者：袁云香 YUAN Yun-Xiang(Key Laboratory for Space Bioscience and Biotechnology,Institute of Special Environmental Biophysics,School of Life Sciences,Northwestern Polytechnical University,Xi’an 710072;College of Environment and Life Sciences,Weinan Normal University,Weinan 714099)

机构地区：[1]西北工业大学生命学院,西北工业大学特殊环境生物物理学研究所,空间生物实验模拟技术国防重点学科实验室,西安710072 [2]渭南师范学院环境与生命科学学院,渭南714099

出　　处：《植物研究》2020年第5期673-678,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31000410);渭南师范学院项目(19HX98);陕西省教育厅科学研究计划项目(18JS036)。

摘　　要：以小果卫矛嫩茎为外植体,采用L 9(34)正交设计法,研究了不同灭菌组合、不同基本培养基、不同浓度6-BA、2,4-D、NAA配比对小果卫矛愈伤组织诱导、再分化及生根的影响。结果表明:小果卫矛嫩茎最适的灭菌组合为75%酒精消毒30 s+0.1%升汞消毒15 min,愈伤组织诱导的最佳培养基为MS+3.0 mg·L-16-BA+1.0 mg·L-12,4-D,诱导率为79%;再分化的最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1,再分化率为78.83%,1/2 MS+NAA 1.2 mg·L-1适用于生根培养,生根率达到83.23%。With the young stems of Euonymus microcarpus(Oliv.)Spraggue as explants,L 9(34)orthogonal test was used to study the effects of different sterilization combinations and different basal medium and combinations of different plant growth regulator on the induction and regeneration and rooting of callus.The most appropriate method of surface sterilization on leaves was with 75%alcohol for 30 s,and then 0.1%HgCl 2 for 15 min,[JP2]the optimal medium for callus induction was MS containing 6-BA 3.0 mg·L-1 and 2,4-D 1.0 mg·L-1[JP]with a maximum induction rate of 79%,the optimum medium for shoot differentiation was MS containing 6-BA 2.0 mg·L-1 and NAA 0.2 mg·L-1 with 78.83%of differentiation rate,and the suitable rooting medium was 1/2MS containing NAA 1.2 mg·L-1 with 83.23%of rooting rate.

关 键 词：小果卫矛 组织培养 愈伤组织 再分化 

分 类 号：Q949.754.7[生物学—植物学]