Kallistatin基因在大鼠阴茎海绵体中的表达研究  

Expression of the kallistatin gene in rat corpus cavernosum

作　　者：转黎 尚坤 官洁[1,2] 李云秀 麦选诚[1,2] 胥琴 ZHUAN Li;SHANG Kun;GUAN Jie;LI Yun-xiu;MAI Xuan-cheng;XU Qin(Department of Reproductive Medicine,The First People's Hospital of Yunnan Province,Kunming,Yunnan 650032,China;Department of Reproductive Medicine,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,Yunnan 650032,China;Medical College of Kunming University of Science and Technology,Kunming,Yunnan 650000,China)

机构地区：[1]云南省第一人民医院生殖医学科,云南昆明650032 [2]昆明理工大学附属医院生殖医学科,云南昆明650032 [3]昆明理工大学医学院,云南昆明650000

出　　处：《中华男科学杂志》2020年第3期223-227,共5页National Journal of Andrology

基　　金：国家自然科学基金项目(81460234,81660247,81760470);云南省科技厅-昆明医科大学应用基础研究联合专项资金项目[2017FE467(-155),2017FE468(-118),2017FE467(-210)];云南省第一人民医院人类辅助生殖技术研究省创新团队(2017HC009);云南省卫生和计划生育委员会医学后备人才培养计划项目(H-201627)。

摘　　要：目的:探讨人组织激肽释放酶结合蛋白(kallistatin)在阴茎海绵体中的表达情况,为勃起功能调控和ED治疗寻找新的分子靶点。方法:采用qRT-PCR、Western印迹和免疫荧光技术检测kallistatin在大鼠阴茎海绵体组织的表达情况,同时检测主动脉中的表达水平进行对照。结果:qRT-PCR和Western印迹检测结果显示,Kallistatin mRNA和蛋白在大鼠阴茎海绵体组织中均有表达,且其表达水平与主动脉相比均无统计学差异(P>0.05);免疫荧光检测显示,Kallistatin表达于阴茎海绵体内皮细胞和平滑肌细胞,且定位于细胞胞质,与主动脉相比,两者间表达亦无明显差异(P>0.05)。结论:Kallistatin基因高表达于阴茎海绵体且定位于海绵体内皮细胞和平滑肌细胞内,提示kallistatin可能在海绵体内皮细胞和平滑肌细胞的细胞功能中发挥作用,从而参与阴茎勃起功能调控。Objective:To investigate the expression of the kallistatin gene in the corpus cavernosum and search for some new molecular targets for the regulation of penile erectile function and treatment of ED.Methods:Using reverse transcriptase-quantitative polymerase chain reaction(RT-qPCR),Western blot and immunofluorescence staining,we detected the expression of kallistatin in the rat corpus cavernosum and compared it with that in the aorta.Results:The results of RT-qPCR and Western blot revealed both mRNA and protein expressions of kallistatin in the rat corpus cavernosal tissue,with no statistically significant difference from those in the aorta(P>0.05).Immunofluorescence staining showed that kallistatin was expressed in both endothelial and smooth muscle cells in the corpus cavernosum and localized in the cytoplasm,with no statistically significant difference from its expression in the aorta(P>0.05)either.Conclusion:The kallistatin gene is highly expressed in the corpus cavernosum and localized in cavernosal endothelial and smooth muscle cells,suggestive of its involvement in the cellular function of cavernosal endothelial and smooth muscle cells and its participation in the regulation of penile erectile function.

关 键 词：Kallistatin基因 阴茎海绵体 勃起功能 勃起功能障碍 大鼠 

分 类 号：R698.1[医药卫生—泌尿科学]