佳蓉片对LOH大鼠睾丸形态结构与功能改善作用研究  

Improving effect of Jiarong Tablets on the morphological structure and function of the testis in rats with late-onset hypogonadism

作　　者：田园园 李波男 周海亮 周青[2] 周兴[2] 邢俊平[1] TIAN Yuan-yuan;LI Bo-nan;ZHOU Hai-liang;ZHOU Qing;ZHOU Xing;XING Jun-ping(Department of Urology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China;Department of Andrology,The First Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410000,China)

机构地区：[1]西安交通大学第一附属医院泌尿外科,陕西西安710061 [2]湖南中医药大学第一附属医院男科,湖南长沙410007

出　　处：《中华男科学杂志》2020年第3期237-241,共5页National Journal of Andrology

基　　金：国家自然科学基金(81202706、81673984);湖南省自然科学基金(2018JJ3402);长沙市杰出创新青年人才项目(kq1802015);湖南省高层次卫生人才225工程医学学科骨干人才培养对象(湘卫函[2019]196号);湖南创新型省份建设专项经费资助-“湖湘青年英才”支持计划(2019RS2041);中华中医药学会“青年人才托举工程”(2019-QNRC2-C07)。

摘　　要：目的:探讨佳蓉片对迟发性性腺功能减退症(LOH)大鼠睾丸形态结构与功能改善作用。方法:18月龄雄性SD大鼠随机分为模型组、佳蓉片组,2月龄雄性SD大鼠设为正常对照组,佳蓉片组给予0.375 g/(kg·d)体重剂量的佳蓉片灌胃,每天1次;正常对照组和模型组给予相同体积的蒸馏水灌胃,连续给药28 d。实验结束后对所有大鼠称重,取血清检测睾酮(T)含量,睾丸组织进行病理学及电镜检测。结果:与正常对照组相比,佳蓉片组、模型组大鼠睾丸系数显著降低(P均<0.05),模型组大鼠血清T水平:[(3.40±0.06)vs(5.88±0.46)ng/ml]显著降低(P<0.05);与模型组相比,佳蓉片组体重和睾丸系数差异无统计学意义(P>0.05),血清T[(4.50±0.78)ng/ml]显著升高(P<0.05)。佳蓉片组大鼠睾丸组织生精小管内精子数量增多,睾丸间质增多明显;电镜示线粒体数量明显增多,部分可见线粒体鞘,部分线粒体嵴清晰,线粒体水肿不明显。结论:佳蓉片能提高LOH大鼠T水平,改善大鼠睾丸组织形态和超微结构。Objective:To investigate the effect of Jiarong Tablets(JRT)on the testicular morphology and function of rats with late-onset hypogonadism(LOH).Methods:LOH models were established in 8 eighteen-month-old male SD rats,treated intragastrically with distilled water(the model control group,n=4)or JRT at 0.375 g/kg/d,qd(the JRT group,n=4),and another 5 twomonth-old normal male SD rats were also given distilled water by gavage(normal control group),all for 28 days.Then all the rats were weighed and sacrificed for measurement of the serum T level and pathological and electron microscopic examination of the testis tissue.Results:Compared with the normal controls,the LOH models showed significantly decreased testis coefficient(P<0.05)and serum T level([3.40±0.06]vs[5.88±0.46]ng/ml,P<0.05).No statistically significant differences were observed in the model control and JRT groups in the body weight and testis coefficient(P>0.05),but the serum T level([4.50±0.78]ng/ml)was remarkably decreased in the latter(P<0.05).In comparison with the model controls,the rats treated with JRT exhibited increases in the sperm count in the seminiferous tubules and the amount of testicular interstitial cells.Electron microscopy revealed a markedly increased number of mitochondria in the JRT-treated animals,with some mitochondrial sheaths and cristae but no obvious mitochondrial edema.Conclusion:Jiarong Tablets can elevate the serum T level and improve the testicular morphology and ultrastructure of LOH rats.

关 键 词：佳蓉片 迟发性性腺功能减退症 睾酮 组织形态 超微结构 大鼠 

分 类 号：R698.2[医药卫生—泌尿科学]