过表达肝/骨/肾型碱性磷酸酶对小鼠急性心肌梗死后心室重塑的影响  

Effect of alkaline phosphatase liver/bone/kidney overexpression on ventricular remodeling after acute myocardial infarction in mice

作　　者：王立友 张冬颖[1] WANG Li-you;ZHANG Dong-ying(Department of Cardiovascular Medicine,the First Affliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院心血管内科,重庆400016

出　　处：《解剖学报》2020年第3期431-436,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(31800976,81970203);中国心血管健康联盟研究院“进阶”研究基金(2018-CCA-AF-034)。

摘　　要：目的探讨肝/骨/肾型碱性磷酸酶(ALPL)过表达对小鼠急性心肌梗死(MI)后心室重塑的影响。方法36只8周龄雄性C57BL/6 J小鼠随机分为对照组(sham组),心肌梗死组(MI+Adv-EGFP组)和ALPL过表达组(MI+Adv-ALPL组),每组12只。术后2周,使用心脏彩色超声测定小鼠心脏功能,HE染色观察小鼠心脏组织病理变化,Real-time PCR检测小鼠心脏ALPL mRNA表达水平,Western blotting检测ALPL、α平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(ColⅠ)和Ⅲ型胶原蛋白(ColⅢ)表达情况,苦味酸天狼猩红染色偏振光法测定各组标本胶原容积分数(CVF)和Ⅰ型/Ⅲ型胶原比值。结果与MI组相比,过表达ALPL组小鼠心脏左室射血分数(LVEF)及左室短轴缩短率(LVFS)明显下降(P<0.05),过表达ALPL组HE染色可见心肌纤维断裂,排列紊乱,梗死区域纤维化明显,心脏组织α-SMA、ColⅠ、ColⅢ蛋白表达及CVF均显著增大(P<0.05),同时ColⅠ/ColⅢ比值显著增加(P<0.05)。结论ALPL过表达加重小鼠急性心肌梗死后心室病理性重塑。Objective To investigate the effect of alkaline phosphatase liver/bone/kidney(ALPL)overexpression on ventricular remodeling after acute myocardial infarction(MI).Methods Thirty six 8-week-old male C57BL/6 J mice were randomly divided into sham group(sham),MI group(MI+Adv-EGFP)and ALPL overexpression group(MI+Adv-ALPL)with 12 mice in each group.Two weeks after MI,cardiac function of mice was detected by echocardiography,pathological changes was detected by HE staining.ALPL mRNA expression in mouse heart was detected by Real-time PCR.The levels of ALPL,α-smooth muscle actin(α-SMA),collagenⅠ(ColⅠ)and collagenⅢ(ColⅢ)protein were detected by Western blotting.The collagen volume fraction(CVF)and the ratio of ColⅠ/ColⅢwere measured by polarizing method with Sirius red staining.Results Compared with MI+Adv-EGFP group,the left ventricular ejection fraction(LVEF)(%)and left ventricular fractional shortening(LVFS)(%)of the heart in MI+Adv-ALPL group were reduced significantly(P<0.05).Hearts in the ALPL overexpression group showed myocardial fiber broken,and disordered and fibrous scars were obvious in myocardial infarction area.The expression of ALPL and the expression ofα-SMA,ColⅠand ColⅢprotein in MI+Adv-ALPL group increased significantly(P<0.05),and the proportion of ColⅠ/ColⅢin MI+Adv-ALPL group increased significantly(P<0.05).Compared with MI+Adv-EGFP group,the CVF and the proportion of the ratio of ColⅠ/ColⅢin the MI+Adv-ALPL group increased significantly(P<0.05).Conclusion ALPL overexpression can promote the ventricular pathological remodeling after acute myocardial infarction in mice.

关 键 词：肝/骨/肾型碱性磷酸酶 心肌梗死 心室重塑 Ⅰ型胶原 Ⅲ型胶原 腺病毒转染 实时定量聚合酶链反应 免疫印迹法 小鼠 

分 类 号：R541[医药卫生—心血管疾病]