基于转录组测序分析NaCl胁迫下新疆野苹果叶和根糖酵解相关基因的表达  

Analysis of genes related to glycolysis in the leaves and roots of Malus sieversii under NaCl stress based on transcriptome sequencing

作　　者：何晨晨 刘俐君 鲁晓燕[1] HE Chenchen;LIU Lijun;LU Xiaoyan(College of Agriculure/Xinjiang Production and Construction Corps Key Laboratory of Special Fruit Sand Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi University,Shihezi 832000,Xinjiang,China)

机构地区：[1]石河子大学农学院·特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832000

出　　处：《果树学报》2020年第7期951-961,共11页Journal of Fruit Science

基　　金：国家自然科学基金委NSFC-新疆联合基金(培育)项目(U1703116)。

摘　　要：【目的】筛选新疆野苹果响应NaCl胁迫相关基因。【方法】以新疆野苹果组培苗为试验材料,对150 mmol∙L^-1 NaCl处理48 h后的新疆野苹果幼苗叶片及根系进行转录组测序。【结果】与对照相比,NaCl处理48 h时,新疆野苹果幼苗叶片中差异表达基因为3364个,其中1745个DEGs在盐胁迫响应中上调表达,1619个DEGs下调表达;根系中差异表达基因为3808个,其中1057个DEGs在盐胁迫响应中上调表达,2751个DEGs下调表达。新疆野苹果叶片和根系中所共有的差异基因有2095个得到注释,这些基因共涉及44个Pathway,富集最显著的Pathway主要有糖酵解/糖异生、磷酸戊糖途径、果糖和甘露糖代谢、丙酮酸代谢等。【结论】通过转录组测序和qRT-PCR验证发现,新疆野苹果受到NaCl胁迫后,在糖酵解过程中TPI、FBPase、pckA、PPDK等基因表达量发生明显变化,说明糖酵解途径在新疆野苹果应答NaCl胁迫过程中起着一定的作用。【Objective】Malus sieversii belongs to the genus Malus of Rosaceae,which has played an important role in the history of apple cultivation in the world.It is widely used as a good rootstock for apple production in Northwest China because of its excellent characters of cold resistance,drought resistance,pest resistance and barren resistance.Therefore,in-depth study on these precious germplasm resources can not only promote rational development and utilization of M.sieversii resources,but also provide abundant gene resources for genetic improvement of Malus genus.Salt tolerance is a complex trait controlled by multi-genes,which involves many physiological and biochemical processes,such as growth,photosynthesis and energy metabolism,osmoregulation and cell signaling.At present,studies on Malus sieversii are mainly seen in the distribution,origin,evolution,and characteristics of physiology and biochemistry.The research on M.sieversii under NaCl stress is mainly seen in the determination of leaf anatomical structure,proline,malondialdehyde,chlorophyll and soluble sugar content,and the research on molecular mechanism is rarely reported.This study analyzed the genes related to glycolysis pathway of M.sieversii under NaCl stress by transcriptome sequencing technology,in order to provide reference for the mining of salt tolerance genes of apple.【Methods】M.sieversii seeds were purchased from Yili,Xinjiang.The seedlings were obtained by tissue culture.After rooting,the uniform seedlings with fine roots were transferred into the water culture boxes with continuous supplyof the Japanese Garden Nutrient Solution.When the 6th to 8th leaves of hydroponic seedlings were fully expanded,the following treatments were carried out:(1)CK,the Japanese Garden Nutrient Solution;(2)NaCl treatment,adding 150 mmol∙L^-1 NaCl to the Japanese Garden Nutrient Solution.In order to avoid salt shock reaction,the concentration of NaCl gradually increased with a gradient of 50 mmol·L^-1 per day,and all the treatments reached the target conce

关 键 词：新疆野苹果 NACL胁迫 转录组测序 糖酵解 

分 类 号：S661.1[农业科学—果树学]