采用UPLC-MS/MS技术分析‘枸头橙’针刺和枝代谢物  

Comparative metabolomics analysis of thorn and branch of‘Goutou’sour orange based on UPLC-MS/MS technique

作　　者：孙立方 柯甫志[1] 聂振朋[1] 王平[1] 孙建华[1] 徐建国[1] SUN Lifang;KE Fuzhi;NIE Zhenpeng;WANG Ping;SUN Jianhua;XU Jianguo(Zhejiang Citrus Research Institute,Zhejiang Branch of National Center for Citrus Variety Improvement,Taizhou 318026,Zhejiang,China)

机构地区：[1]浙江省柑橘研究所·国家柑橘品种改良中心浙江分中心,浙江台州318026

出　　处：《果树学报》2020年第7期962-970,共9页Journal of Fruit Science

基　　金：国家现代农业(柑橘)产业技术体系岗位科学家项目(CARS-26);浙江省农业(果品)新品种选育重大科技专项(2016C2052-1);浙江省农业科学院扶持学科(柑橘育种)项目;台州优势水果产业技术创新团队项目。

摘　　要：【目的】明确柑橘针刺和枝的差异代谢物,探究二者差异发育的调控因素,为深入研究针刺发育机制提供依据。【方法】以‘枸头橙’(Citrus aurantium L.‘Goutou’Sour Orange)为材料,采用石蜡切片技术分析针刺和枝内部组织结构差异;利用超高效液相色谱—串联质谱(UPLC-MS/MS)和广泛靶向代谢组技术检测并筛选针刺和枝的差异代谢物。【结果】柑橘枝内表皮细胞存在分泌道、叶或侧枝原基细胞,而针刺中则无。共筛选到针刺和枝的差异显著代谢物138种,枝中上调代谢物81种,下调代谢物57种,以苯丙素类、黄酮类、萜类、生物碱类和多酚类等次生代谢物为主。差异代谢物注释到74条通路上,主要富集通路包括代谢通路、次生代谢物合成和黄酮(黄酮醇)类合成等路径。【结论】柑橘针刺和枝内部组织结构存在明显差异,明确了二者之间以次生代谢物为主的差异显著代谢物及其主要注释通路,表明次生代谢物在针刺和枝的差异发育中起着重要调控作用,为深入研究针刺发育机制奠定了一定的基础。【Objective】Most of the thorns and branches of citrus initially formed in the same position of leaf axil,and finally developed into the thorns and branches separately,indicating that there were different regulation mechanisms of development between them.However,little is known about the molecular mechanism of the thorn development of citrus.In a previous study,we found that the differentially expressed genes between the thorn and branch of citrus mainly focused on the pathways related to the synthesis of secondary metabolites.In order to further elucidate the regulatory factors of the differential development of the thorn and branch,the differences of the internal structure and metabolites between the thorn and branch were investigated,which would provide the clues and basis for further study of the regulation mechanism of the thorn development.【Methods】The top parts of the young thorns and branches(1 cm in length)were collected from the leaf axils of the new shoots from 5-year-old‘Goutou’Sour Orange seedlings.The differences of the internal structure of the thorn and branch were observed and analyzed using the paraffin section method.The differential metabolites between the thorn and branch were detected and screened by UPLC-MS/MS and widely targeted metabolome technology.The metabolome data were analyzed based on MWDB(metware database)and OPLS-DA(orthogonal signal correction and partial least squares-discriminant analysis)model,and the VIP(variable import in project)and fold change values were used to screen the differential metabolites between the thorn and branch.Statistical pathway enrichment analysis of the differential metabolites was performed based on the KEGG database.【Results】The results of cross sections of the thorn and branch showed that there were obvious differences between the internal structure of the thorn and branch.There were secretory canal,leaf or lateral branch primordium in the epidermal cells of the branch,but not in the thorn.However,the other internal structures of the t

关 键 词：柑橘 ‘枸头橙’ 针刺 枝 代谢组 次生代谢物 

分 类 号：S666.4[农业科学—果树学]