桑根酮D对肿瘤细胞及移植瘤生长作用研究  

Effect of sanggenon D on the growth of tumor cells and transplantation tumors

作　　者：王艺 魏华 杨光 张梅 WANG Yi;WEI Hua;YANG Guang;ZHANG Mei(Key Laboratory of Plant Medicine Resources Utilization of Ministry of Education in Xinjiang,School of Pharmacy Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学药学院新疆植物药资源利用教育部重点实验室,新疆石河子832000

出　　处：《天然产物研究与开发》2020年第6期1057-1064,共8页Natural Product Research and Development

基　　金：石河子大学高层次人才项目(KCZX201532)。

摘　　要：研究桑根酮D的体内外抗肿瘤作用。采用的体外实验,桑根酮D溶液体外共培养三种癌细胞(人肝癌HepG2细胞、小鼠黑色素瘤B16F0细胞、小鼠黑色素瘤B16F10细胞),采用SRB法(Sulforhodamine B)检测桑根酮D对肿瘤细胞增殖的影响,计算IC50。对黑色素瘤细胞(B16F0和B16F10细胞)给予不同浓度的桑根酮D,考察其对黑色素含量的影响。采用的体内实验,建立小鼠H22肿瘤模型并分组,三个剂量的桑根酮D分别为12.5、25、50 mg/kg,考察其对肿瘤生长的抑制作用。结果显示,桑根酮D抑制B16F0、B16F10、HepG2细胞增殖,HepG2细胞对桑根酮D最敏感,得IC50为22.61±0.26μmol/L。随着桑根酮D浓度的增加,抑制细胞的作用增强且均有显著性差异(P<0.01)。桑根酮D促进B16F0细胞内黑色素生成,促进细胞分化。12.5、25、50 mg/kg剂量的桑根酮D对小鼠肝癌细胞H22移植瘤抑制率为32.51%~45.72%。给药组小鼠的体重及各脏器指数变化与空白对照组和模型组比较均无显著性差异。得出结论,桑根酮D具有体内外抗肝癌作用和促进肿瘤细胞分化的作用。To study the anti-tumor effect of sanggenon D in vivo and in vitro.The in vitro method was first to co-culture three types of cancer cells(human liver cancer HEPG2 cells,mouse melanoma B16F0 cells,and mouse melanoma B16F10 cells)with sanggenon D solution.Then,SRB method(sulforhodamine B)was used to investigate the effect of sanggenon D on the proliferation of tumor cells,and IC 50 was calculated.The effects of different concentrations of sanggenon D on melanoma cells were investigated.The in vivo experimental method was to establish a mouse H22 tumor model,and to investigate the inhibitory effect of the drug on tumor growth by intraperitoneal injection of 12.5,25 and 50 mg/kg of sanggenon D.The results showed that sanggenon D inhibited the proliferation of B16F0,B16F10 and HepG2 cells,among which HepG2 cells were the most sensitive to sanggenon D,and IC 50 was calculated to be 22.61±0.26μmol/L The inhibitory effect on the cells was enhanced with the increase of the concentration of sanggenon D,and the difference was significant(P<0.01).sanggenon D promotes melanin production in B16F0 cells and promotes cell differentiation.The inhibitory rate of H22 transplanted tumor in mice at 12.5,25 and 50 mg/kg was 32.51%to 45.72%.There was no significant difference in the changes of body weight and organ index in the mice treated with sanggenon D compared with the blank group and the model group.This indicated that sanggenon D could inhibit the growth of transplanted tumor and the proliferation of tumor cells in mice,and it also indicated that sanggenon D had anti-tumor effects in vivo and in vitro.

关 键 词：桑根酮D 肿瘤细胞 H22移植瘤 体内体外抗癌活性 

分 类 号：R961[医药卫生—药理学]