诱导细胞凋亡DFF45样效应因子C的结构与表达调控及其功能  

Structural,Expression and Functional Analysis of Cell Death-inducing DNA Fragmentation Factor 45-like Effector C(CIDEC)

作　　者：李红强 郭振清 杜金友 LI Hong-Qiang;GUO Zhen-Qing;DU Jin-You(College of Agriculture and Biotechnology,Hebei Normal University of Science&Technology,Biotechnology teaching department,ChangLi 066600,Hebei,China)

机构地区：[1]河北科技师范学院农学与生物科技学院生物技术教学部,河北昌黎066600

出　　处：《中国生物化学与分子生物学报》2020年第5期512-518,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.31702101);河北省高等学校科学研究青年基金项目(No.QN2016136);河北省人才培养工程资助项目(No.A201901055)资助。

摘　　要：诱导细胞凋亡DFF45样效应因子C(cell death-inducing DNA fragmentation factor 45-like effector C,CIDEC)是近几年发现的一种脂滴结合蛋白质,属于CIDE家族成员,具有N端和C端两个结构域。该基因的组织分布具有明显的差异性,主要在脂肪组织和肝中高表达。高脂饮食和禁食等营养条件也会引起该基因表达量增高。该基因的表达受到多种因素的调控,包括多种转录因子和营养相关因子。近些年来发现,CIDEC定位于脂滴表面,调控脂滴间的融合。进一步研究发现该蛋白质与其他蛋白质(例如CIDEC,CIDEA、PLIN1)相互作用形成复合体引起接触的脂滴之间形成孔道。随后,脂质从小脂滴转运到大脂滴,进而使脂滴之间发生融合和生长。另外发现,CIDEC是促进细胞凋亡的重要蛋白质,同时在抑制脂肪分解方面具有重要作用。本文重点介绍CIDEC的结构特点,转录调控机制、亚细胞定位以及主要功能的作用机制,并对未来的研究方向提出思考,为CIDEC作用机制的有关研究提供理论基础和探索思路。Cell death-inducing DNA fragmentation factor 45-like effector C(CIDEC)is a newly discovered lipid droplet-binding protein.It is a member of the CIDE family and has both N-terminal and C-terminal domains.The expression of this gene is tissue-specific,with high expression in adipose tissues and livers.In addition,nutritional conditions such as high-fat diets and fasting also increase gene expression.The gene expression is regulated by many factors,including many transcription factors and some nutrition-related factors.In recent years,CIDEC has been found to locate on the surface of lipid droplets and regulate the fusion of lipid droplets.Further studies have found that it interacts with other proteins(such as CIDEC,CIDEA and PLIN1)to form complexes,resulting in the formation of pores between contacting lipid droplets,followed by the transport of lipids from small lipid droplets to large lipid droplets,which then leads to the fusion and growth of lipid droplets.In addition,CIDEC promotes apoptosis and plays an important role in inhibiting fat decomposition.This paper focuses on the structural features of CIDEC,transcriptional regulation mechanism,subcellular localization and the mechanism of its main functions,and puts forward some thoughts on the future research directions,which will provide a theoretical basis to further explore CIDEC’s mechanism of action.

关 键 词：诱导细胞凋亡DFF45样效应因子C 脂滴 表达调控 生物学功能 

分 类 号：Q71[生物学—分子生物学]