辛伐他汀联合雌二醇对去卵巢大鼠骨质疏松的影响  

Effect of simvastatin combined with estradiol on osteoporosis in ovariectomized rats

作　　者：李泽清[1] 丁军稳 任荣[1] 丁启龙[2] 王哲 米涛 陆川[1] 李钊伟[1] LI Zeqing;DING Junwen;REN Rong;DING Qilong;WANG Zhe;MI Tao;LU Chuan;LI Zhaowei(Affiliated Hospital of Qinghai University, Xining 810000, China;The Second People’s Hospital of Huangzhong County, Xining 811601)

机构地区：[1]青海大学附属医院,青海西宁810000 [2]青海省湟中县第二人民医院,青海西宁811601

出　　处：《中国骨质疏松杂志》2020年第6期838-842,共5页Chinese Journal of Osteoporosis

基　　金：青海省基础研究计划项目(2018-0302-ZJC-0042)。

摘　　要：目的探讨辛伐他汀联合雌二醇对大鼠骨质疏松的影响。方法75只雌性SD大鼠随机分为5组:假手术组、去势组、辛伐他汀组、雌二醇组、联合药物组,15只/组,构建去卵巢大鼠骨质疏松模型,给予不同药物干预。检测骨代谢相关生化指标及骨生物力学指标,测定骨组织骨密度(bone mineral density,BMD)和骨矿物含量(bone mineral content,BMC),HE染色观察骨组织形态结构。结果去势组血清骨代谢相关生化指标(ALP、BGP、PICP、TRAP)水平较假手术组均显著增高(P<0.05),辛伐他汀组、雌二醇组、联合药物组较去势组均降低,尤以联合用药组降低最显著(P<0.05)。去势组骨组织BMD和BMC、骨生物力学指标(最大载荷、最大应力、最大位移和刚度)较假手术组显著降低(P<0.05),辛伐他汀组、雌二醇组、联合药物组较去势组均增高,尤以联合药物组增高最显著(P<0.05)。去势组骨组织骨小梁减少稀疏,排列紊乱,网状结构破坏,大量纤维组织,髓腔内大量空泡状脂肪细胞。联合药物组骨组织结构较完整,骨小梁数目增多,致密均匀粗壮,连接成网状结构,脂肪细胞明显减少。结论辛伐他汀联合雌二醇可调节大鼠骨代谢,增加骨密度和骨矿物质,改善骨生物力学和骨组织形态学,起到抗骨质疏松疗效和骨保护作用。Objective To study the effect of simvastatin combined with estradiol on osteoporosis in rats.Methods Seventy-five female SD rats were randomly divided into 5 groups:sham operation group,castration group,simvastatin group,estradiol group,and combined drug group,with 15 rats in each group.Ovariectomized rat osteoporosis model was established.Different drug intervention was applied.Bone metabolism related biochemical indicators and bone biomechanical indicators were detected.Bone mineral density(BMD)and bone mineral content(BMC)were determined.Bone morphological structure was observed with HE staining.Results The biochemical parameters of serum bone metabolism(ALP,BGP,PICP,TRAP)in the ovariectomized group were significantly higher than those in the sham operation group(P<0.05).They were lower in simvastatin group,the estradiol group,and the combination group than in the castrated group.They were the lowest in the combination group(P<0.05).BMD,BMC,and bone biomechanical parameters(maximum load,maximum stress,maximum displacement and stiffness)in the ovariectomized group were significantly lower than those in the sham operation group(P<0.05).They were higher in simvastatin group,the estradiol group,and the combination group than in the castrated group.The increase was most significant in the combination group(P<0.05).The trabecular bone in the ovariectomized group was sparsely arranged and disordered.Reticular structure,massive fibrous tissue,and many vacuolar fat cells appeared in the medullary cavity.In the combined drug group,a complete bone structure appeared.The number of trabecular bone increased,showing dense,uniform,and a network structure.The fat cells reduced significantly.Conclusion Simvastatin combined with estradiol regulates bone metabolism,increases BMD and bone minerals,improves bone biomechanics and bone histomorphology,and plays an anti-osteoporosis and bone protection role.

关 键 词：辛伐他汀 雌二醇 去势大鼠 骨质疏松 

分 类 号：R318[医药卫生—生物医学工程] R-332[医药卫生—基础医学]