雌二醇对去卵巢骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响  

Effects of estradiol on Wnt/β-catenin signaling pathway in bone tissue of ovariectomized osteoporosis rats

作　　者：邱敏[1] 翟书珩 付勤[1] QIU Min;ZHAI Shuheng;FU Qin(Department of Orthopedics,Shengjing Hospital of China Medical University,Shenyang 063000,China)

机构地区：[1]中国医科大学附属盛京医院骨科,辽宁沈阳110004

出　　处：《中国骨质疏松杂志》2020年第6期857-861,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察雌二醇对去卵巢骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响,探讨其防治绝经后骨质疏松症的作用机制。方法将80只雌性SD大鼠随机分为对照组、假手术组、去势组、药物组,构建绝经后骨质疏松大鼠模型,检测骨组织骨密度(bone mineral density,BMD)和生物力学指标,运用qRT-PCR和免疫组化分别检测骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达,通过HE染色观察骨组织形态学。结果去势组股骨BMD、生物力学指标较对照组和假手术组均显著降低(P<0.05),药物组股骨BMD、生物力学指标较去势组均显著升高(P<0.05)。去势组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较对照组和假手术组均显著降低(P<0.05),药物组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较去势组均显著升高(P<0.05)。去势组骨小梁明显减少,排列稀疏不规则,连接不完整,骨髓腔变大,有大量纤维组织。药物组骨小梁数量减少不明显,粗细均匀稍致密,排列尚规则,连续性完整性较好。结论雌二醇通过Wnt/β-catenin信号通路作用于BMP-2,增加了去卵巢骨质疏松大鼠骨密度,提高了骨生物力学性能,能够改善骨组织结构,发挥了抗骨质疏松作用。Objective To investigate the effect of estradiol on Wnt/β-catenin signaling pathway in ovariectomized osteoporosis rats,and to explore its mechanism of prevention and treatment of postmenopausal osteoporosis.Methods 80 female SD rats were randomly divided into control group,sham operation group,castration group and drug group.Construct postmenopausal osteoporosis rat models.Detection of bone tissue BMD and biomechanical indicators,qRT-PCR and immunohistochemistry were respectively used to detect mRNA and protein expression of Wnt,β-catenin and BMP-2 in bone tissue.Results Compared with the control group and the sham operation group,the BMD and biomechanical indexes of the castration group were significantly reduced(P<0.05),Compared with the castration group,the BMD and biomechanical indexes of the drug group were significantly increased(P<0.05).Compared with the control group and the sham operation group,The mRNA and protein expressions of Wnt,β-catenin and BMP-2 of the castration group were significantly reduced(P<0.05),Compared with the castration group,The mRNA and protein expressions of Wnt,β-catenin and BMP-2 of the drug group were significantly increased(P<0.05).The trabecular bone in the castration group was significantly reduced,the arrangement was sparse and irregular,the connection was incomplete,the bone marrow cavity became large,and a large amount of fibrous tissue.The number of trabecular bone in the drug group was not significantly reduced,the thickness was even and dense,the arrangement was regular,and the continuity integrity was good.Conclusion Estradiol acts on BMP-2 via Wnt/β-catenin signaling pathway,increases bone mineral density in ovariectomized osteoporosis rats,improves bone biomechanical properties,improves bone structure,and plays an anti-osteoporosis effects.

关 键 词：雌二醇 骨质疏松症 分泌型糖蛋白/β-连环蛋白 骨形态发生蛋白-2 

分 类 号：R318[医药卫生—生物医学工程]