病毒性脑炎患儿外周血miR-146a、miR-146b表达检测及临床意义  

Detection of peripheral blood miR-146a and miR-146b levels in children with viral encephalitis and their clinical significance

作　　者：魏从真[1] 赵小洁[1] 李彦明[1] 柴越 边策 赵帅[1] WEI Congzhen;ZHAO Xiaojie;LI Yanming;CHAI Yue;BIAN Ce;ZHAO Shuai(The First Clinical Laboratory,the First Hospital of Hebei Medical University,Hebei Province,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第一医院检验一科,河北石家庄050000

出　　处：《中国医药导报》2020年第17期96-100,共5页China Medical Herald

基　　金：河北省医学科学研究重点课题计划项目(20150189)。

摘　　要：目的检测病毒性脑炎患儿外周血miR-146a、miR-146b的表达,并探讨其临床意义。方法回顾性分析2016年6月~2019年6月河北医科大学第一医院(以下简称"我院)收治的62例病毒性脑炎患儿的临床资料和同期在我院体检的43名健康儿童的资料,分别记为研究组和对照组。根据感染病毒类型将研究组进一步分为研究A组(单纯疱疹病毒7例)、研究B组(带状疱疹病毒9例)、研究C组(肠道病毒28例)、研究D组(腮腺病毒6例)、研究E组(流行性乙型病毒12例)5个亚组。比较研究组、对照组及研究组各亚组中轻型和重型的miR-146a、miR-146b水平;比较研究组和对照组T淋巴细胞亚群水平,分析研究组外周血miR-146a、miR-146b与T淋巴细胞亚群的相关性,比较不同预后患儿外周血miR-146a、miR-146b水平。结果不同研究组亚组和研究组miR-146a、miR-146b均高于对照组(P <0.05),且重型患儿均高于轻型患儿(P <0.05);不同研究组和研究组亚组CD+3、CD+4及CD+4/CD+8均低于对照组(P <0.05),而CD+8均高于对照组(P <0.05);不同研究组和研究组亚组miR-146a、miR-146b与CD+3、CD+4、及CD+4/CD+8均呈负相关(P <0.05),与CD+8均呈正相关(P <0.05);研究组预后不良率为17.74%,预后不良患儿miR-146a、miR-146b均高于预后良好者(P <0.05)。结论病毒性脑炎患儿外周血miR-146a、miR-146b的表达水平均升高,能够反映病情并与T淋巴细胞亚群具有相关性,且可以预测预后情况。Objective To detect the peripheral blood miR-146 a and miR-146 b levels in children with viral encephalitis,and to explore their clinical significance.Methods Clinical data of 62 children with viral encephalitis admitted to the First Hospital of Hebei Medical University("our hospital"for short)from June 2016 to June 2019 and the physical examination data of 43 healthy subjects during the same period in our hospital were retrospectively analyzed,and they were recored as study group and control group respectively.The study group was further divided into 5 subgroups according to the virus type:study group A(7 cases of herpes simplex virus),study group B(9 cases of herpes zoster virus),study group C(28 cases of enterovirus),study group D(6 cases of parotid virus),and study group E(12 cases of epidemic virus B).The levels of miR-146 a and miR-146 b between the study group,the control group and mild and severe cases of subgroups of study group were compared.The levels of T lymphocyte sub-population were compared between the study group and the control group.The correlations between the levels of miR-146 a and miR-146 b in peripheral blood and T lymphocyte sub-population levels in study group were analyzed.The levels of miR-146 a and miR-146 b in peripheral blood of different prognosis were compared.Results The levels of miR-146 a and miR-146 b in the study group and different sub-groups of the study group were higher than those in the control group(P<0.05),of which the severe cases were higher than the mild cases(P<0.05).The CD+3,CD+4 and CD+4/CD+8 in the study group and different sub-groups of the study group were lower than the control group(P<0.05),while the CD+8 levels were higher than the control group(P<0.05).The levels of miR-146 a and miR-146 b of the study group and different sub-groups of the study group were negatively correlated with CD+3,CD+4,and CD+4/CD+8(P<0.05),which were positively correlated with CD+8(P<0.05).The levels of miR-146 a and miR-146 b in children with good prognosis were significantly lowe

关 键 词：病毒性脑炎 MIR-146A miR-146b T淋巴细胞亚群 

分 类 号：R512.3[医药卫生—内科学]