单细胞转录组测序技术在类风湿性关节炎的研究进展  

Research progress of single cell RNA sequencing technology in rheumatoid arthritis

作　　者：李琼[1] 陈宇婷 接力刚 毋静[3] 杜红延 LI Qiong;CHEN Yuting;JIE Ligang;WU Jing;DU Hongyan(First College of Clinical Medicine,Southern Medical University,Guangzhou,Guangdong,China,510515;School of Laboratory Medicine and Biotechnology,Southern Medical University,Guangzhou,Guangdong,China,510515;Department of Rheumatology and Clinical Immunology,Zhujiang Hospital,Southern Medical University,Guangzhou,Guangdong,China,510282)

机构地区：[1]南方医科大学第一临床医学院,广东广州510515 [2]南方医科大学检验与生物技术学院,广东广州510515 [3]南方医科大学珠江医院检验科,广东广州510282

出　　处：《分子诊断与治疗杂志》2020年第6期835-838,共4页Journal of Molecular Diagnostics and Therapy

基　　金：国家自然科学基金(81601397,81102688);广东省自然科学基金(2016A030313624)。

摘　　要：类风湿性关节炎(RA)是常见的自身免疫性疾病,由于其参与的细胞种类众多发病机制复杂,目前仍然机制未明。单细胞研究技术的出现为从细胞基础上研究免疫耐受的破坏和促炎通路失调提供了新的手段,并可能从根本上改变对风湿病发病机制的分子通路的理解。单细胞转录组测序(sc RNAseq)可以解决普通转录组技术不能精确到细胞类型的问题,对组织中的细胞进行独立分析,提供无偏倚的结果,为细胞亚群的鉴定提供更多mRNA信息。这项技术在RA的广泛应用为展示滑膜细胞的异质性,鉴定致病细胞群以及揭示疾病发生发展的机制提供了有力的工具。本文将主要围绕目前sc RNA-seq在RA中的研究进展进行阐述sc RNA-seq的原理和常用的测序平台以及在RA研究中所取得的成果。The pathogenesis of rheumatoid arthritis(RA),one of the most prevalent autoimmune diseases,is still unknown due to its complex disease-associated cell composition and function.Technological advances are enabling the analysis of single cell data,which could provide researchers with opportunities to study the breakdown of immune tolerance and dysregulated pro-inflammatory pathways on a cell-by-cell basis,and fundamentally change our understanding of the molecular pathways that underpin the pathogenesis of rheumatic diseases.Single cell RNA-sequencing(sc RNA-seq)can reduce the bias that bulk RNA-seq cannot be accurate to cell type by performing independent sequencing of every cell within a tissue,and provide more transcriptome information for cell clustering.The wide application of this technology in RA also provides a powerful tool for identifying synovial cell heterogeneity and pathogenic cell groups,and revealing the mechanism of disease.This article focuses on the current research progress of sc RNA-seq in RA,including the basic principle,common platforms,and achievements in RA.

关 键 词：单细胞转录组测序 类风湿性关节炎 细胞亚群鉴定 细胞异质性 

分 类 号：R593.22[医药卫生—内科学]