定向冷冻明胶支架的原位细菌纤维素培养及支架中的细胞行为  

In situ bacterial cellulose culture and cell behavior in directional frozen gelatin scaffolds

作　　者：江雨 吴擢彤 王宝秀 王华平[1] 李喆[1] Jiang Yu;Wu Zhuotong;Wang Baoxiu;Wang Huaping;Li Zhe(State Key Laboratory of Fiber Material Modification, School of Materials Science and Engineering, Donghua University, Shanghai 201620, China)

机构地区：[1]东华大学材料科学与工程学院,纤维材料改性国家重点实验室,上海201620

出　　处：《合成技术及应用》2020年第2期5-8,38,共5页Synthetic Technology & Application

摘　　要：本文以明胶(G)为原料,采用定向冷冻干燥法制备出定向明胶支架,通过原位培养技术在支架引入细菌纤维素(BC)纳米纤维网络,构筑出兼具微米多孔和纳米纤维结构的BC基定向微/纳支架,并使用场发射扫描电镜(FE-SEM)、激光共聚焦显微镜(CLSM)等对支架的微观结构生物学性能进行了表征。结果表明:与非定向支架的多孔结构相比,定向支架具有径向多孔和轴向通道结构,呈现各向异性,这种通道结构有利于氧气和营养物质的输送,能有效促进细胞生长。In this paper,gelatin was used as raw material to prepare directional gelatin scaffold by directional freeze-drying method.Bacterial cellulose(BC)based directional micro/nano scaffold with micro porous and nano fiber structure was constructed by introducing BC nanofiber network into the scaffold through in-situ culture technology.The microstructure and biological properties of the scaffold were characterized by field emission scanning electron microscopyand laser confocal microscopy.The results showed that compared with the porous structure of the non-directional scaffold,the directional scaffold had radial porous and axial channel structure,showing anisotropy.This channel structure is conducive to the transport of oxygen and nutrients,and can effectively promote cell growth.

关 键 词：组织工程 细菌纤维素 定向 冷冻干燥法 原位培养 

分 类 号：TQ352[化学工程]