东北对开蕨孢子离体快繁技术研究  

Study on in vitro rapid propagation of the spore of Potentilla anserina from Northeast China

作　　者：韩汶雨 刘建星 徐钰童 陈浩宇 于海阔 刘畅 李余先 建德锋 HAN Wenyu;LIU Jianxing;XU Yutong;CHEN Haoyu;YU Haikuo;LIU Chang;LI Yuxian;JIAN Defeng(College of Traditional Chinese Medicine,Jilin Agricultural Science and Technology University Jilin,Jilin 132109)

机构地区：[1]吉林农业科技学院中药学院,吉林吉林132109

出　　处：《现代农业研究》2020年第6期38-39,共2页Modern Agriculture Research

基　　金：吉林省大学生创新创业项目([2019]002)。

摘　　要：该实验以东北对开蕨的孢子为外植体进行离体培养试验,得出了东北对开蕨孢子离体快繁的途径:孢子试管培养初步得出原叶体,原叶体通过扩繁数量增多,然后将这些原叶体进行试管外诱导得到孢子体,培养孢子体即可得到东北对开蕨的植株。另通过配方比较得出:试管内采用MS培养基增殖效果最好,试管外培养过程中定期喷施0.1%KH2PO4诱导剂效果最好。The experiment to the northeast divided fern spores of explant in vitro culture experiment was car⁃ried out,it is concluded that the northeast divided fern spores in vitro rapid propagation path:preliminary draw prothallus spores in vitro,prothallus through propagation quantity increased,and then will be in vitro in⁃duced to get these prothallus sporophyte,cultivate sporophyte obtains the northeast divided fern plants.In ad⁃dition,formula comparison showed that MS medium had the best effect of proliferation in vitro and 0.1%KH2PO4 inducer was sprayed regularly in vitro.

关 键 词：孢子 东北对开蕨 原叶体增殖 孢子体诱导 

分 类 号：S682.35[农业科学—观赏园艺]