匹伐他汀对高尿酸血症大鼠炎症因子及氧化应激的影响  

Effects of pivastatin on inflammatory factors and oxidative stress in rats with hyperuricemia

作　　者：尤杨[1] 夏岳[1] 戚国庆[1] 高宏阳[1,2] 杨志瑜[1] 李柳[1] 赵红亮[1] YOU Yang;XIA Yue;QI Guo-Qing;GAO Hong-Yang;YANG Zhi-Yu;LI Liu;ZHAO Hong-Liang(Department of Cardiology,First Hosipital of Hebei Medical University,Shijiazhuang 050031,China)

机构地区：[1]河北医科大学第一医院心内三科,石家庄050031 [2]河北医科大学第一医院骨二科,石家庄050031

出　　处：《中国免疫学杂志》2020年第11期1299-1303,共5页Chinese Journal of Immunology

基　　金：河北省科技厅重点研发计划项目(18277742D)资助。

摘　　要：目的:探讨匹伐他汀对高尿酸血症大鼠氧化应激指标和炎症因子水平的影响。方法:将36只SD大鼠随机分为对照组、模型组和实验组,每组12只。模型组和实验组大鼠灌胃给予酵母膏和腺嘌呤建立高尿酸血症大鼠模型。实验组大鼠灌胃给予匹伐他汀0.3 mg/kg,对照组与模型组大鼠灌胃给予等体积生理盐水,连续给药4周。HE染色观察大鼠肾脏组织病理变化情况,分析各组大鼠血清尿酸(UA)、超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和谷胱肽过氧化物酶(GSH-Px)水平,检测血清中IL-6、肿瘤坏死因子(TNF-α)和转化生长因子-β1(TGF-β1)的水平。结果:与对照组比较,模型组大鼠肾小球和肾小管上皮细胞肿胀,毛细血管袢有结节状玻璃样物质沉积,纤维组织增多,血清UA水平明显升高(P<0.05),MAD含量明显升高(P<0.05),SOD、T-AOC活性明显降低(P<0.05);IL-6、TNF-α和TGF-β1水平明显升高(P<0.05);与模型组比较,实验组大鼠肾小球和肾小管上皮细胞肿胀程度改善;血清UA水平明显降低(P<0.05);血清MAD含量明显降低(P<0.05),血清SOD、T-AOC活性明显升高(P<0.05),GSH-Px和CAT活力差异无统计学意义(P>0.05);血清IL-6、TNF-α和TGF-β1水平明显降低(P<0.05)。结论:匹伐他汀能够控制UA水平,抑制炎症因子分泌,减轻高尿酸血症大鼠体内氧化应激。Objective:To investigate effect of pivastatin on the levels of oxidative stress and inflammatory factors in rats with hyperuricemia.Methods:36 SD rats were randomly divided into control group,model group and experimental group,12 rats in each group.Rats in model group and experimental group were given yeast extract and adenine by gavage to establish a rat model of hyperuricemia.Rats in experimental group were given 0.3 mg/kg of pivastatin by gavage,while rats in control group and model group were given qual volumes of normal saline by gavage for 4 weeks.HE staining was used to observe pathological changes of kindey tissues of rate.Levels of serum uric acid(UA),superoxide dismutase(SOD),malondialdehyde(MDA),total antioxidant capacity(T-AOC),catalase(CAT)and glutathione peroxidase(GSH-Px)were analyzed.Levels of IL-6,tumor necrosis factor(TNF-α)and transforming growth factor-β1(TGF-β1)were detected.Results:Compared with control group,glomerular and tubular epithelial cells in model group were swollen,hyaline depositions were found in capillary nodular,fibrous tissues were increased,and level of serum UA was significantly increased(P<0.05).Content of MAD increased significantly(P<0.05),and activity of SOD and T-AOC decreased significantly(P<0.05).IL-6,TNF-αand TGF-β1 levels were significantly increased(P<0.05).Compared with model group,degree of swelling of glomerular and tubular epithelial cells in experimental group was improved,level of serum UA was significantly decreased(P<0.05),content of serum MAD was significantly decreased(P<0.05),levels of serum SOD and T-AOC activity were significantly increased(P<0.05),and GSH-Px and CAT activity were not significantly different(P>0.05),levels of serum of IL-6,TNF-αand TGF-β1 were significantly decreased(P<0.05).Conclusion:Pivastatin can control level of UA,inhibit secretion of inflammatory factors,and reduce oxidative stress in hyperuricemia rats.

关 键 词：匹伐他汀 高尿酸血症 氧化应激 炎症因子 

分 类 号：R589.7[医药卫生—内分泌]