MitoKATP通道在异氟醚对急性肺损伤保护作用中的影响  

Effect of MitoKATP channel on protective effect of isoflurane on acute lung injury

作　　者：韩晓丹 孙敏莉 HAN Xiao-dan;SUN Min-li(Department of Anesthesia, Zhongshan Hospital, Fudan University, Shanghai 200032, China)

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032

出　　处：《中国临床医学》2020年第3期510-514,共5页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨MitoKATP通道在异氟醚预处理对急性肺损伤(acute lung injury,ALI)保护作用中的影响。方法:将30头5~6周龄、9~14 kg雄性幼猪随机分成5组(n=6),分别为对照组、脂多糖组(LPS组)、异氟醚组(ISO组)、5-羟基癸酸盐(5-hydroxydecanoate,5-HD)组(HD组)和HD-ISO组,分别静脉输注生理盐水、静脉输注内毒素、吸入异氟醚预处理后静脉输注内毒素、静脉输注5-HD预处理后静脉输注内毒素、静脉输注5-HD和吸入异氟醚预处理后静脉输注内毒素。ALI形成后观察外周血白细胞(WBC)的动态变化、肺泡灌洗液(broncho-alveolar lavage fluid,BALF)中总磷脂(total phospholipid,TPL)含量、肺组织IL-8 mRNA和ICAM-1 mRNA表达。结果:与对照组相比,LPS组在ALI形成时、ALI 2 h、4 h时WBC均明显下降(P<0.05),肺组织IL-8 mRNA、ICAM-1 mRNA表达明显增加,BALF中TPL显著下降(P<0.05),H-E染色示大量炎性细胞浸润。与LPS组相比,ISO组在ALI 4 h时WBC显著增加,肺组织IL-8 mRNA、ICAM-1 mRNA表达明显下降,BALF中TPL显著增加(P<0.05),H-E染色示炎性细胞浸润明显减少。与ISO组相比,HD-ISO组在ALI 2 h和4 h时WBC显著减少,肺组织IL-8 mRNA、ICAM-1 mRNA表达明显增加;BALF中TPL显著下降(P<0.05),H-E染色示炎性细胞浸润明显增加。结论:异氟醚预处理可能通过MitoKATP通道来抑制ALI时炎症因子和细胞黏附分子的释放。Objective:To explore the effects of MitoK ATP channel on the protective effect of isoflurane pretreatment on acute lung injury induced by endotoxin in piglets.Methods:Thirty young piglets of 5-6 weeks old,weighing 9-14 kg were anesthetized,intratracheally intubated,and underwent mechanical ventilation.The animals were divided into 5 groups(n=6 each group):control group,lipopolysaccharide(LPS)group,isoflurane(ISO)group,5-hydroxydeconate(HD)group,and HD-ISO group.Control group was pretreated by normal saline,and LPS group was pretreated by endotoxin.ISO group was pretreated by alveolar concentrations(MAC)isoflurane,HD group was pretreated by 5-HD,HD-ISO group was pretreated by 5-HD,isoflurane,each group was pretreated and then injected endotoxin.After the establishment of acute lung injury model,the animals were killed and lungs were processed for measurement of peripheral white blood cell(WBC),the interleukin(IL)-8 and intercellular cell adhesion molecule(ICAM)-1 mRNA level,total phospholipid content in bronchoalveolar lavage fluid(BALF).Results:Compared with control group,WBC was decreased at ALI 0 h,2 h,and 4 h in LPS group after ALI induction,IL-8 mRNA and ICAM-1 mRNA were increased,the total phospholipid content in BALF was decreased(P<0.05),hematoxylin-eosin(HE)staining showed that there was significant inflammatory cell infiltration in the lung tissue.Compared with LPS group,WBC was increased at 4 h in ISO group after ALI induction,IL-8 mRNA and ICAM-1 mRNA were decreased,the total phospholipid content in BALF was increased(P<0.05),HE staining showed less inflammatory cellular infiltration.Compared with ISO group,WBC was decreased 2 h and 4 h in HD-ISO group after ALI induction,IL-8 mRNA and ICAM-1 mRNA were increased,the total phospholipid content in BALF was decreased(P<0.05),H-E staining showed more inflammatory cell infiltration.Conclusions:Inhalation of isoflurane may inhibit the release of inflammatory factors and cell adhesion factors in piglets with acute lung injury through MitoK ATP channel.

关 键 词：异氟醚 MitoKATP通道 急性肺损伤 IL-8 ICAM-1 

分 类 号：R563[医药卫生—呼吸系统]