NaCl胁迫对马铃薯试管苗POD酶活性及同工酶的影响  

Effects of NaCl Stress on POD Enzyme Activity and Isozyme of Potato Tube Plantlets

作　　者：裴怀弟[1] 刘润萍[2] 林玉红[1] 李淑洁[1] 吕汰[3] PEI Huaidi;LIU Runping;LIN Yuhong;LI Shujie;Lv Tai(Institute of Biotechnology,Gansu Academy of Agriculture Sciences,Lanzhou Gansu 730070,China;Institute of Agricultural Econamic and Information,Gansu Academy of Agriculture Sciences,Lanzhou Gansu 730070,China;Tianshui Institute of Agricultural sciences,Tianshui Gansu 741000,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]甘肃省农业科学院农业经济与信息研究所,甘肃兰州730070 [3]天水市农业科学研究所,甘肃天水741000

出　　处：《甘肃农业科技》2020年第6期12-15,共4页Gansu Agricultural Science and Technology

基　　金：国家自然科学基金地区基金项目(31960599);现代农业产业技术体系(CARS-107);甘肃省农业科学院重点研发计划(2020GAAS13)。

摘　　要：选用4个马铃薯品种(系)的试管苗,在不同NaCl浓度的培养基上诱导分化成苗,对其耐盐性进行初步研究。结果表明,随着NaCl浓度的增加,大西洋、L03-1、L03-6的POD酶活性与无胁迫处理相比呈现下降趋势,但L03-2与无胁迫处理相比POD酶活性呈增加趋势。采用聚丙烯酰胺凝胶电泳对NaCl胁迫马铃薯试管苗POD同工酶监测表明,马铃薯盐诱导再生苗POD同工酶谱带存在品种(系)间显著差异,盐胁迫已经不能使POD同工酶谱带保持完整,参试马铃薯品种(系)对盐浓度敏感程度存在差异。In this experiment,the tube plantlets of four potato cultivars(lines)were seleted,Directly induce to differentiate into seedlings on medium containing different NaCl concentrations,preliminary identification of its salt tolerance.The results showed that with the increase of NaCl concentration,the POD enzyme activity of Atlantic,L03-1 and L03-6 showed a decreasing trend compared with treatment without stress,but the POD enzyme activity of L03-2 showed an increasing trend compared with the treatment.Monitoring of POD isoenzymes in NaCl stress potato tube plantlets by polyacrylamide gel electrophoresis showed that the potato salt-induced seedling of POD isozyme bands existed significant differences,salt stress could not maintain the integrity of the POD isozymes.In this experiment four potato varieties on the salt sensitivity presenced difference.

关 键 词：马铃薯试管苗 盐胁迫 POD酶活性 同工酶 

分 类 号：S513[农业科学—作物学]