Differential Expression and Regulation of Switchgrass Root MicroRNA in Response to Alkali-salt Stress Using High-throughput Deep Sequencing  

作　　者：Liu Jian Wang Qian-nan Guo Xin Duo Tian-qi Li Ran Hu Guo-fu Zhang Xun-zhong 

机构地区：[1]College of Animal Sciences and Technology,Northeast Agricultural University,Harbin150030,China [2]School of Plant and Environmental Science,Virginia Tech,Blacksburg,VA,USA,24061

出　　处：《Journal of Northeast Agricultural University(English Edition)》2020年第2期81-89,共9页东北农业大学学报（英文版）

基　　金：Supported by the Natural Science Fund of Heilongjiang Province(LC2016009)(GH);U.S.Department of Energy and U.S.Department of Agricultural Plant Feedstocks Genomics for Bioenergy Program(DE-SC0008338 to XZ and BZ)。

摘　　要：Switchgrass(Panicum virgatum L.)as a high-quality bioenergy crop that can effectively improve saline-alkali soil has strong resistance to stress and grows well in marginal soil and some abiotic stress environments.This study used alkali-sensitive genotype AM(AM-314/MS-155)and alkali-tolerant genotype ALA(Alamo)as experimental materials to investigate molecular mechanisms of switchgrass tolerance to alkali-salt stress.When the plants were grown to E5 stage,the alkali-salt stress treatment was carried out by soaking method(Na2CO3:NaHCO3=1:9,C(Na+)=150 mmol·L-1 and pH=9.0)and fresh root samples were taken after treatments for 0(CK),6 and 24 h,respectively,the differentially expressed microRNAs and their regulatory network were analyzed.A total of 1049 known miRNAs and 68 novel miRNAs were identified.Seventy-two differentially expressed miRNAs in ALA were more than three times higher than those in AM and 36.1%differentially expressed miRNAs was significantly down-regulated(p<0.05).Through analyses of differentially expressed miRNAs and their target genes,it was found that under alkali-salt stress,differentially expressed miRNAs in AM were mainly involved in the regulation of cellular ROS clearance,ethylene signal transduction,and root,leaf and flower development.MiRNAs in ALA were also involved in water transport,DNA methylation,response to high osmotic pressure,activation of stress-related genes and more complex responses to alkali-salt stress processes,but those in AM were not.ALA was significantly higher than AM in the number of microRNAs responding to alkali-salt stress and in the functional diversity of their regulatory target genes.

关 键 词：miRNA alkali-salt stress high-throughput sequencing SWITCHGRASS 

分 类 号：S543[农业科学—作物学]