鳜Follistatin基因cDNA的克隆及其胚胎发育表达分析  

Cloning and Embryonic Developmental Expression Analysis of Follistatin Gene in Siniperca chuatsi

作　　者：胡姜丽 徐蕾[1] 许艺兰 刘晶洁 孙悦 谢炎东 宾石玉[1,2] HU Jiangli;XU Lei;XU Yilan;LIU Jingjie;SUN Yue;XIE Yandong;BIN Shiyu(College of Life Science, Guangxi Normal University, Guilin Guangxi 541006, China;Key Laboratory of Ecologyof Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministsry of Education,Guilin Guangxi 541006, China)

机构地区：[1]广西师范大学生命科学学院,广西桂林541006 [2]珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学),广西桂林541006

出　　处：《广西师范大学学报（自然科学版）》2020年第4期124-131,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31560718)。

摘　　要：为探讨鳜Siniperca chuatsi卵泡抑素(Follistatin,FST)基因在胚胎发育时期的表达状况,本文采用qRT-PCR、3′RACE和5′RACE技术从鳜胚胎组织中克隆得到鳜FST基因的全长cDNA序列(GenBank登陆号:KM077175.1)为1271 bp。生物信息学分析表明,鳜FST基因编码区为960 bp,编码319个氨基酸。同时,对鳜FST氨基酸序列与其他物种的同源性以及系统进化特征进行了分析。qRT-PCT定量分析证实,FST在鳜胚胎发育早期的受精期和卵裂期开始低量表达,之后从囊胚期表达开始显著性增加,在神经胚期达到最高表达,随后FST表达保持较低表达水平。In order to investigate the expression of Follistatin(FST)gene in Siniperca chuatsi during embryonic development,the full-length cDNA sequence of FST gene was cloned by qRT-PCR,3′RACE and 5′RACE techniques from the embryonic tissue of S.chuatsi(GenBank:KM077175.1).Bioinformatics analysis showed that the full length of cDNA is 1271 bp with coding region 960 bp and encoding 319 amino acids.The amino acid sequence identity of FST and their phylogenetical relations with other species were also assayed.The expression profile of FST was analyzed with qRT-PCR at different embryonic development stages in S.chuatsi.The results showed that FST expression is relatively low at both fertilization and cleavage stages.After that,its expression increases significantly from blastocyst stage,reaches the highest at neural stage,and then its expression remains at relative low level.

关 键 词：鳜 FST基因 克隆 胚胎发育 表达分析 

分 类 号：Q959.483[生物学—动物学] S917.4[农业科学—水产科学]