苯并15-冠-5为受体的双光子荧光钠离子探针  

Sodium-ions-selective two-photon fluorescent probe with benzo-15-crown-5 as acceptor

作　　者：黄池宝[1] 陈小勇 蒲浩[1] 吕国岭 HUANG Chibao;CHEN Xiaoyong;PU Hao;LU Guoling(Qianbei Information Technology Research Institute,Zunyi Normal University,Zunyi 563002,Guizhou,China)

机构地区：[1]遵义师范学院黔北信息技术研究院,贵州遵义563002

出　　处：《精细化工》2020年第6期1158-1162,共5页Fine Chemicals

基　　金：国家自然科学基金(21562050);贵州省科技支撑计划项目(“防控新型冠状病毒技术攻关及集成应用”项目,黔科合支撑[2020]4Y001号);贵州省高层次创新型人才培养计划项目——“百”层次人才(黔科合平台人才【2016】5683);贵州省第八批科技创新人才团队建设专项基金(黔科合人才团队[2015]4007);贵州省科学技术基金项目(黔科合J字[2015]2146);贵州省教育厅自然科学重点研究项目(黔教合KY字[2014]296);贵州省教育厅自然科学研究项目(黔教合KY字[2019]068)。

摘　　要：以苯并15-冠-5和双氰基二苯代乙烯分别作为钠离子受体与双光子荧光团开发出探针(DNa)。采用核磁共振谱和质谱对DNa的结构进行了确证,同时对其光物理性能进行了表征。探针DNa具有小的分子尺寸、大的双光子吸收截面(δTPA,1054GM)、极好的光稳定性、适宜的水溶性和优良的细胞渗透性。DNa对Na^+显示优良的专一选择性,不受其他离子的干扰,对Na^+的解离常数Kd=(23±2)mmol/L。DNa能用于细胞中Na^+的三维成像,是性能优良的双光子荧光探针。A novel two-photon fluorescent probe(DNa)for Na+detection was developed using benzo-15-crown-5 and dicyanostilbene as Na+ligand and two-photon fluorophore,respectively.The structure of DNa was identified by NMR spectroscopy and mass spectrometry.At the same time,its photophysical properties were characterized.Probe DNa possessed small molecule size,large two-photon absorption cross-section(δTPA,1054 GM),excellent photostability,suitable water solubility,and good cell permeability.Probe DNa showed excellent selectivity to Na^+,and was not interfered by other ions.Its dissociation constant Kd for Na^+was(23±2)mmol/L.Proble DNa was able to be used for three-dimensional imaging of Na+in cells.So,it is a two-photon fluorescent probe with excellent performance.

关 键 词：双光子荧光探针 钠离子 双氰基二苯代乙烯 双光子吸收 细胞成像 功能材料 

分 类 号：O657.3[理学—分析化学]