鼠巨细胞病毒感染小鼠胎盘病理学的改变对小鼠子代宫内生长发育情况的影响  

Effect of the placental pathological changes of murine cytomegalovirus infected mice on the intrauterine growth and development of mice offspring

作　　者：龚琦 陈素华 马超 魏蕾 张志军[2] GONG Qi;CHEN Suhua;MA Chao;WEI Lei;ZHANG Zhijun(Postgraduate Training Base of Jinzhou Medical University,Taihe Hospital of Shiyan City(Affiliated Hospital of Hubei University of Medicine),Hubei Province,Shiyan 442000,China;Center for Reproductive Medicine,Affiliated Hospital of Hubei University of Medicine,Hubei Province,Shiyan 442000,China;Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong Science and Technology University,Hubei Province,Wuhan 430030,China)

机构地区：[1]锦州医科大学十堰市太和医院研究生培养基地(湖北医药学院附属医院),湖北十堰442000 [2]湖北医药学院附属太和医院生殖医学中心,湖北十堰442000 [3]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国医药导报》2020年第16期20-23,I0004,共5页China Medical Herald

基　　金：湖北省卫生和计划生育委员会青年人才项目(WJ2017Q036)。

摘　　要：目的观察鼠巨细胞病毒(MCMV)感染小鼠胎盘病理学改变对子代宫内生长发育情况的影响。方法采用酶联免疫吸附法,在性成熟期小鼠中选取未感染MCMV的雌鼠,进行合笼受孕。采用随机数字表法将小鼠分为对照组与模型组,各8只。在孕龄12.5 d时,模型组透过胎盘进行MCMV悬液显微注射,对照组注射等量培养基溶液;在孕龄18.5 d时,断颈法处死雌鼠,观察98只胎鼠各个器组织标本病理改变。结果模型组胎盘感染率和胎仔感染率明显高于对照组,差异有统计学意义(P<0.05)。有胎盘感染小鼠中胎仔感染率明显高于无胎盘感染者,差异有统计学意义(P<0.05);在有胎盘感染小鼠中,有细胞滋养细胞增生、合体细胞结节增生及纤维素样坏死者发生死胎和异常胎仔率明显高于无细胞滋养细胞增生、合体细胞结节增生及纤维素样坏死者,差异均有统计学意义(P<0.05);有纤维素样坏死者畸胎率明显高于无纤维素样坏死者,差异有统计学意义(P<0.05)。结论经胎盘感染MCMV是导致宫内感染的重要途径,MCMV感染是导致子代不良妊娠结局重要原因。Objective To observe the efficacy of pathological placental changes after intrauterine murine cytomegalovirus(MCMV)infection on the intrauterine growth and development of fetal mice.Methods The mature mice without MCMV infection identified by enzyme-linked immunoabsorbent assay were served as the subjects.The male and the female mice were put into the same cage.According to the method of random number table,the mice were randomly divided into control group and model group,with 8 mice in each group.At day 12.5 of gestation,the placentas of the model group were injected with MCMV,while the control group was injected with cell maintenance medium.All female mice were executed at day 18.5 of gestation,and the change of tissues and organs of 98 fetal mice were observed.Results The placental infection rate and fetal infection rate in the model group were significantly higher than those in the control group,the differences were statistically significant(P<0.05).In the placenta infection mice,the fetal infection rate increased significantly compared with those without placenta infection,the difference was statistically significant(P<0.05).In the placenta infection mice,the rates of stillbirths and abnormal fetuses in mice with cytotrophoblast proliferative,syncytial trophoblast nodules and fibrinoid necrosis were significance higher than those without cytotrophoblast proliferative,syncytial trophoblast nodules and fibrinoid necrosis,the differences were statistically significant(P<0.05).The teratogenic rate in mice with fibrinoid necrosis was significantly higher than those without fibrinoid necrosis,the difference was statistically significant(P<0.05).Conclusion The MCMV infection through placenta is an important approach for intrauterine infection.MCMV infection is an important cause of adverse pregnancy outcomes in offspring.

关 键 词：巨细胞病毒 小鼠 胎盘感染 病理学 生长发育 

分 类 号：R373.9[医药卫生—病原生物学]